With increasing interest in biohydrogen as an alternative fuel to petroleum, it is essential to identify a hyper biohydrogen producer. In the present study, Clostridium sp. YD09 was isolated from a brewery wastewater upflow anaerobic sludge blanket digestion reactor; its 16S ribosomal RNA sequencing analysis showed 96 % similarity to Clostridium beijerinckii. Clostridium sp. YD09 produced the highest cumulative volume of hydrogen using xylose as the substrate (optimal concentration of 10 g/L) to obtain 1.21 mol H2/mol xylose. Furthermore, Clostridium sp. YD09 tolerates various inhibitors from pre-treated lignocellulosic biomass up to a 0.1 % concentration. A maximum yield of 1.62 mol H2/mol xylose and 1.98 mL H2/mL media with 38- to 48-fold increase was recorded in ligno-hemicellulose xylose treated with biochar and activated carbon. The newly isolated Clostridium sp. YD09 can therefore be used for efficient biohydrogen production from xylose-based substrates.
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© 2022 Elsevier B.V.
All Science Journal Classification (ASJC) codes
- Environmental Engineering
- Biomedical Engineering