Hyper biohydrogen production from xylose and xylose-based hemicellulose biomass by the novel strain Clostridium sp. YD09

Sang Hyun Kim, Ye Da Yi, Hyun Joong Kim, Shashi Kant Bhatia, Ranjit Gurav, Jong Min Jeon, Jeong Jun Yoon, Sang Hyoun Kim, Jeong Hoon Park, Yung Hun Yang

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

With increasing interest in biohydrogen as an alternative fuel to petroleum, it is essential to identify a hyper biohydrogen producer. In the present study, Clostridium sp. YD09 was isolated from a brewery wastewater upflow anaerobic sludge blanket digestion reactor; its 16S ribosomal RNA sequencing analysis showed 96 % similarity to Clostridium beijerinckii. Clostridium sp. YD09 produced the highest cumulative volume of hydrogen using xylose as the substrate (optimal concentration of 10 g/L) to obtain 1.21 mol H2/mol xylose. Furthermore, Clostridium sp. YD09 tolerates various inhibitors from pre-treated lignocellulosic biomass up to a 0.1 % concentration. A maximum yield of 1.62 mol H2/mol xylose and 1.98 mL H2/mL media with 38- to 48-fold increase was recorded in ligno-hemicellulose xylose treated with biochar and activated carbon. The newly isolated Clostridium sp. YD09 can therefore be used for efficient biohydrogen production from xylose-based substrates.

Original languageEnglish
Article number108624
JournalBiochemical Engineering Journal
Volume187
DOIs
Publication statusPublished - 2022 Nov

Bibliographical note

Publisher Copyright:
© 2022 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Environmental Engineering
  • Biomedical Engineering

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