Hydrogen and ethanol fermentation of various carbon sources by immobilized Escherichia coli (XL1-Blue)

Periyasamy Sivagurunathan, Gopalakrishnan Kumar, Chiu Yue Lin

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38 Citations (Scopus)


In this study, hydrogen and ethanol production by a facultative anaerobic bacterium Escherichia coli XL1-Blue immobilized in calcium-alginate beads have been investigated. Batch fermentations were carried out at mesophilic temperature (35 °C) and an initial cultivation pH of 6.5. Firstly, the influence of biomass concentration in terms of dry cell weight (expressed in g DCW/L, range 0.2-1.0) was investigated using fructose (5 g/L) as a carbon source. The peak hydrogen yield (HY) of 1.17 mol-H2/mol-fructose utilized was obtained at an initial cell concentration of 0.4 g DCW/L. The hydrogen production potential of other simple carbon sources (glucose and xylose) was evaluated at this optimized cell concentration and peak HY values were attained as 0.96 mol-H2/mol-glucoseutilized and 0.69 mol-H2/mol-xyloseutilized, respectively. In addition, utilization of the beverage wastewater (BWW) showed the peak cumulative hydrogen production and ethanol concentration of 120 mL and 5.65 g/L, attained at the substrate concentration of 20 g (glucose equivalent)/L. However, peak HY (1.65 mol-H 2/mol-glucose eqivalent utilized) was observed at low substrate concentration of 5 g(glucose equivalent)/L. The percentage of sugar utilization of BWW was ranged between 80 and 96.

Original languageEnglish
Pages (from-to)6881-6888
Number of pages8
JournalInternational Journal of Hydrogen Energy
Issue number13
Publication statusPublished - 2014 Apr 24

Bibliographical note

Funding Information:
The authors gratefully acknowledge the financial support by Taiwan's Bureau of Energy (grant no. 102-D0616 ), Taiwan's National Science Council ( NSC-102-2221-E-035-002-MY3 ), and APEC Research Center for Advanced Biohydrogen Technology ( 102-2911-I-035-001 ), Taiwan. The bacterial strain E. coli XL1-Blue (provided by Dr. Peter Bakonyi, University of Pannonia, Veszprem, Hungary) is gratefully acknowledged.

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


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