Hybrid Enzymatic/Photocatalytic Degradation of Antibiotics via Morphologically Programmable Light-Driven ZnO Microrobots

Cagatay M. Oral; Martina Ussia; Martin Pumera

doi:10.1002/smll.202202600

Hybrid Enzymatic/Photocatalytic Degradation of Antibiotics via Morphologically Programmable Light-Driven ZnO Microrobots

Cagatay M. Oral, Martina Ussia, Martin Pumera

Department of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Antibiotics are antimicrobial substances that can be used for preventive and therapeutic purposes in humans and animals. Their overdose usage has led to uncontrolled release to the environment, contributing significantly to the development of antimicrobial resistance phenomena. Here, enzyme-immobilized self-propelled zinc oxide (ZnO) microrobots are proposed to effectively target and degrade the released antibiotics in water bodies. Specifically, the morphology of the microrobots is tailored via the incorporation of Au during the synthetic process to lead the light-controlled motion into having on/off switching abilities. The microrobots are further modified with laccase enzyme by physical adsorption, and the immobilization process is confirmed by enzymatic activity measurements. Oxytetracycline (OTC) is used as a model of veterinary antibiotics to investigate the enzyme-immobilized microrobots for their removal capacities. The results demonstrate that the presence of laccase on the microrobot surfaces can enhance the removal of antibiotics via oxidation. This concept for immobilizing enzymes on self-propelled light-driven microrobots leads to the effective removal of the released antibiotics from water bodies with an environmentally friendly strategy.

Original language	English
Article number	2202600
Journal	Small
Volume	18
Issue number	39
DOIs	https://doi.org/10.1002/smll.202202600
Publication status	Published - 2022 Sept 28

Bibliographical note

Funding Information:
C.M.O. acknowledges the grant CEITEC‐K‐21‐7049, financed from Quality Internal Grants of BUT (Reg. No. CZ.02.2.69/0.0/0.0/19_073/0016948). M.U. was supported by the ESF under the project CZ.02.2.69/0.0/0.0/18_053/0016962. M.P. was supported by MEYS CR grant LL2002 under ERC CZ program. CzechNanoLab project LM2018110 funded by MEYS CR is gratefully acknowledged for the financial support of the measurements/sample fabrication at CEITEC Nano Research Infrastructure. The authors would like to thank Dr. M. Urso for scientific discussions.

Publisher Copyright:
© 2022 Wiley-VCH GmbH.

All Science Journal Classification (ASJC) codes

Biotechnology
Chemistry(all)
Biomaterials
Materials Science(all)

Access to Document

10.1002/smll.202202600

Cite this

@article{f65ccf84ffcc4a9ca568e5a755910b47,

title = "Hybrid Enzymatic/Photocatalytic Degradation of Antibiotics via Morphologically Programmable Light-Driven ZnO Microrobots",

abstract = "Antibiotics are antimicrobial substances that can be used for preventive and therapeutic purposes in humans and animals. Their overdose usage has led to uncontrolled release to the environment, contributing significantly to the development of antimicrobial resistance phenomena. Here, enzyme-immobilized self-propelled zinc oxide (ZnO) microrobots are proposed to effectively target and degrade the released antibiotics in water bodies. Specifically, the morphology of the microrobots is tailored via the incorporation of Au during the synthetic process to lead the light-controlled motion into having on/off switching abilities. The microrobots are further modified with laccase enzyme by physical adsorption, and the immobilization process is confirmed by enzymatic activity measurements. Oxytetracycline (OTC) is used as a model of veterinary antibiotics to investigate the enzyme-immobilized microrobots for their removal capacities. The results demonstrate that the presence of laccase on the microrobot surfaces can enhance the removal of antibiotics via oxidation. This concept for immobilizing enzymes on self-propelled light-driven microrobots leads to the effective removal of the released antibiotics from water bodies with an environmentally friendly strategy.",

author = "Oral, {Cagatay M.} and Martina Ussia and Martin Pumera",

note = "Funding Information: C.M.O. acknowledges the grant CEITEC‐K‐21‐7049, financed from Quality Internal Grants of BUT (Reg. No. CZ.02.2.69/0.0/0.0/19_073/0016948). M.U. was supported by the ESF under the project CZ.02.2.69/0.0/0.0/18_053/0016962. M.P. was supported by MEYS CR grant LL2002 under ERC CZ program. CzechNanoLab project LM2018110 funded by MEYS CR is gratefully acknowledged for the financial support of the measurements/sample fabrication at CEITEC Nano Research Infrastructure. The authors would like to thank Dr. M. Urso for scientific discussions. Publisher Copyright: {\textcopyright} 2022 Wiley-VCH GmbH.",

year = "2022",

month = sep,

day = "28",

doi = "10.1002/smll.202202600",

language = "English",

volume = "18",

journal = "Small",

issn = "1613-6810",

publisher = "Wiley-VCH Verlag",

number = "39",

}

TY - JOUR

T1 - Hybrid Enzymatic/Photocatalytic Degradation of Antibiotics via Morphologically Programmable Light-Driven ZnO Microrobots

AU - Oral, Cagatay M.

AU - Ussia, Martina

AU - Pumera, Martin

N1 - Funding Information: C.M.O. acknowledges the grant CEITEC‐K‐21‐7049, financed from Quality Internal Grants of BUT (Reg. No. CZ.02.2.69/0.0/0.0/19_073/0016948). M.U. was supported by the ESF under the project CZ.02.2.69/0.0/0.0/18_053/0016962. M.P. was supported by MEYS CR grant LL2002 under ERC CZ program. CzechNanoLab project LM2018110 funded by MEYS CR is gratefully acknowledged for the financial support of the measurements/sample fabrication at CEITEC Nano Research Infrastructure. The authors would like to thank Dr. M. Urso for scientific discussions. Publisher Copyright: © 2022 Wiley-VCH GmbH.

PY - 2022/9/28

Y1 - 2022/9/28

N2 - Antibiotics are antimicrobial substances that can be used for preventive and therapeutic purposes in humans and animals. Their overdose usage has led to uncontrolled release to the environment, contributing significantly to the development of antimicrobial resistance phenomena. Here, enzyme-immobilized self-propelled zinc oxide (ZnO) microrobots are proposed to effectively target and degrade the released antibiotics in water bodies. Specifically, the morphology of the microrobots is tailored via the incorporation of Au during the synthetic process to lead the light-controlled motion into having on/off switching abilities. The microrobots are further modified with laccase enzyme by physical adsorption, and the immobilization process is confirmed by enzymatic activity measurements. Oxytetracycline (OTC) is used as a model of veterinary antibiotics to investigate the enzyme-immobilized microrobots for their removal capacities. The results demonstrate that the presence of laccase on the microrobot surfaces can enhance the removal of antibiotics via oxidation. This concept for immobilizing enzymes on self-propelled light-driven microrobots leads to the effective removal of the released antibiotics from water bodies with an environmentally friendly strategy.

AB - Antibiotics are antimicrobial substances that can be used for preventive and therapeutic purposes in humans and animals. Their overdose usage has led to uncontrolled release to the environment, contributing significantly to the development of antimicrobial resistance phenomena. Here, enzyme-immobilized self-propelled zinc oxide (ZnO) microrobots are proposed to effectively target and degrade the released antibiotics in water bodies. Specifically, the morphology of the microrobots is tailored via the incorporation of Au during the synthetic process to lead the light-controlled motion into having on/off switching abilities. The microrobots are further modified with laccase enzyme by physical adsorption, and the immobilization process is confirmed by enzymatic activity measurements. Oxytetracycline (OTC) is used as a model of veterinary antibiotics to investigate the enzyme-immobilized microrobots for their removal capacities. The results demonstrate that the presence of laccase on the microrobot surfaces can enhance the removal of antibiotics via oxidation. This concept for immobilizing enzymes on self-propelled light-driven microrobots leads to the effective removal of the released antibiotics from water bodies with an environmentally friendly strategy.

UR - http://www.scopus.com/inward/record.url?scp=85136983962&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85136983962&partnerID=8YFLogxK

U2 - 10.1002/smll.202202600

DO - 10.1002/smll.202202600

M3 - Article

C2 - 36026536

AN - SCOPUS:85136983962

SN - 1613-6810

VL - 18

JO - Small

JF - Small

IS - 39

M1 - 2202600

ER -

Hybrid Enzymatic/Photocatalytic Degradation of Antibiotics via Morphologically Programmable Light-Driven ZnO Microrobots

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this