Hyaluronic acid receptor-targetable imidazolized nanovectors for induction of gastric cancer cell death by RNA interference

Eunjung Kim; Jaemoon Yang; Hyun Ouk Kim; Yonghee An; Eun Kyung Lim; Gyudo Lee; Taeyun Kwon; Jae Ho Cheong; Jin Suck Suh; Yong Min Huh; Seungjoo Haam

doi:10.1016/j.biomaterials.2013.02.006

Hyaluronic acid receptor-targetable imidazolized nanovectors for induction of gastric cancer cell death by RNA interference

Eunjung Kim, Jaemoon Yang, Hyun Ouk Kim, Yonghee An, Eun Kyung Lim, Gyudo Lee, Taeyun Kwon, Jae Ho Cheong, Jin Suck Suh, Yong Min Huh, Seungjoo Haam

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

We have developed a nanovector consisting of hyaluronic acid (HA) and poly- l-lysine- graft-imidazole (PLI)-based polyplexes containing Bcl-xL-specific shRNA-encoding plasmid DNA (HA/PLI/pDNA) for CD44 targeted gastric cancer therapy. The prepared ternary polyplexes have a negative surface charge of -24 mV and a size of approximately 100 nm at an N/P ratio of 5 with HA/PLI molar ratio of 0.03. Gel electrophoresis and cell viability experiments demonstrated that the ternary polyplexes showed high stability and no cytotoxicity due to the anchored HA molecules on the surface of PLI/pDNA binary polyplexes. Selective cancer cell death was achieved by CD44-mediated gene delivery and the internalized gene was effectively escaped from endosomes due to the buffering capacity of imidazole groups in an acidic environment. These nanovectors may be highly efficient gene delivery tools that allow the selective destruction of metastatic gastric cancer cells.

Original language	English
Pages (from-to)	4327-4338
Number of pages	12
Journal	Biomaterials
Volume	34
Issue number	17
DOIs	https://doi.org/10.1016/j.biomaterials.2013.02.006
Publication status	Published - 2013 Jun

Bibliographical note

Funding Information:
This work was supported by National Research Foundation of Korea Grant funded by the Korean Government (MEST) ( 2010-0019923 ), the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MEST) ( 2012050077 ). This study was also supported by a grant from the National R&D Program for Cancer Control, Ministry for Health and Welfare, Republic of Korea ( 1020390 ). We are grateful to Yonsei-Carl Zeiss Advanced Imaging Center, Yonsei University College of Medicine, for technical assistance.

All Science Journal Classification (ASJC) codes

Biophysics
Bioengineering
Ceramics and Composites
Biomaterials
Mechanics of Materials

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.biomaterials.2013.02.006

Cite this

@article{83f1f6bceceb42b99345456f789d94ea,

title = "Hyaluronic acid receptor-targetable imidazolized nanovectors for induction of gastric cancer cell death by RNA interference",

abstract = "We have developed a nanovector consisting of hyaluronic acid (HA) and poly- l-lysine- graft-imidazole (PLI)-based polyplexes containing Bcl-xL-specific shRNA-encoding plasmid DNA (HA/PLI/pDNA) for CD44 targeted gastric cancer therapy. The prepared ternary polyplexes have a negative surface charge of -24 mV and a size of approximately 100 nm at an N/P ratio of 5 with HA/PLI molar ratio of 0.03. Gel electrophoresis and cell viability experiments demonstrated that the ternary polyplexes showed high stability and no cytotoxicity due to the anchored HA molecules on the surface of PLI/pDNA binary polyplexes. Selective cancer cell death was achieved by CD44-mediated gene delivery and the internalized gene was effectively escaped from endosomes due to the buffering capacity of imidazole groups in an acidic environment. These nanovectors may be highly efficient gene delivery tools that allow the selective destruction of metastatic gastric cancer cells.",

author = "Eunjung Kim and Jaemoon Yang and Kim, {Hyun Ouk} and Yonghee An and Lim, {Eun Kyung} and Gyudo Lee and Taeyun Kwon and Cheong, {Jae Ho} and Suh, {Jin Suck} and Huh, {Yong Min} and Seungjoo Haam",

note = "Funding Information: This work was supported by National Research Foundation of Korea Grant funded by the Korean Government (MEST) ( 2010-0019923 ), the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MEST) ( 2012050077 ). This study was also supported by a grant from the National R&D Program for Cancer Control, Ministry for Health and Welfare, Republic of Korea ( 1020390 ). We are grateful to Yonsei-Carl Zeiss Advanced Imaging Center, Yonsei University College of Medicine, for technical assistance. ",

year = "2013",

month = jun,

doi = "10.1016/j.biomaterials.2013.02.006",

language = "English",

volume = "34",

pages = "4327--4338",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier BV",

number = "17",

}

TY - JOUR

T1 - Hyaluronic acid receptor-targetable imidazolized nanovectors for induction of gastric cancer cell death by RNA interference

AU - Kim, Eunjung

AU - Yang, Jaemoon

AU - Kim, Hyun Ouk

AU - An, Yonghee

AU - Lim, Eun Kyung

AU - Lee, Gyudo

AU - Kwon, Taeyun

AU - Cheong, Jae Ho

AU - Suh, Jin Suck

AU - Huh, Yong Min

AU - Haam, Seungjoo

N1 - Funding Information: This work was supported by National Research Foundation of Korea Grant funded by the Korean Government (MEST) ( 2010-0019923 ), the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MEST) ( 2012050077 ). This study was also supported by a grant from the National R&D Program for Cancer Control, Ministry for Health and Welfare, Republic of Korea ( 1020390 ). We are grateful to Yonsei-Carl Zeiss Advanced Imaging Center, Yonsei University College of Medicine, for technical assistance.

PY - 2013/6

Y1 - 2013/6

N2 - We have developed a nanovector consisting of hyaluronic acid (HA) and poly- l-lysine- graft-imidazole (PLI)-based polyplexes containing Bcl-xL-specific shRNA-encoding plasmid DNA (HA/PLI/pDNA) for CD44 targeted gastric cancer therapy. The prepared ternary polyplexes have a negative surface charge of -24 mV and a size of approximately 100 nm at an N/P ratio of 5 with HA/PLI molar ratio of 0.03. Gel electrophoresis and cell viability experiments demonstrated that the ternary polyplexes showed high stability and no cytotoxicity due to the anchored HA molecules on the surface of PLI/pDNA binary polyplexes. Selective cancer cell death was achieved by CD44-mediated gene delivery and the internalized gene was effectively escaped from endosomes due to the buffering capacity of imidazole groups in an acidic environment. These nanovectors may be highly efficient gene delivery tools that allow the selective destruction of metastatic gastric cancer cells.

AB - We have developed a nanovector consisting of hyaluronic acid (HA) and poly- l-lysine- graft-imidazole (PLI)-based polyplexes containing Bcl-xL-specific shRNA-encoding plasmid DNA (HA/PLI/pDNA) for CD44 targeted gastric cancer therapy. The prepared ternary polyplexes have a negative surface charge of -24 mV and a size of approximately 100 nm at an N/P ratio of 5 with HA/PLI molar ratio of 0.03. Gel electrophoresis and cell viability experiments demonstrated that the ternary polyplexes showed high stability and no cytotoxicity due to the anchored HA molecules on the surface of PLI/pDNA binary polyplexes. Selective cancer cell death was achieved by CD44-mediated gene delivery and the internalized gene was effectively escaped from endosomes due to the buffering capacity of imidazole groups in an acidic environment. These nanovectors may be highly efficient gene delivery tools that allow the selective destruction of metastatic gastric cancer cells.

UR - http://www.scopus.com/inward/record.url?scp=84875506201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875506201&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2013.02.006

DO - 10.1016/j.biomaterials.2013.02.006

M3 - Article

C2 - 23478035

AN - SCOPUS:84875506201

SN - 0142-9612

VL - 34

SP - 4327

EP - 4338

JO - Biomaterials

JF - Biomaterials

IS - 17

ER -

Hyaluronic acid receptor-targetable imidazolized nanovectors for induction of gastric cancer cell death by RNA interference

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this