Human bone marrow stem cells cultured under hypoxic conditions present altered characteristics and enhanced in vivo tissue regeneration

Jung Seok Lee; Jung Chul Park; Tae Wan Kim; Byung Joo Jung; Youngseok Lee; Eun Kyung Shim; Soyon Park; Eun Young Choi; Kyoo Sung Cho; Chang Sung Kim

doi:10.1016/j.bone.2015.04.044

Human bone marrow stem cells cultured under hypoxic conditions present altered characteristics and enhanced in vivo tissue regeneration

Jung Seok Lee, Jung Chul Park, Tae Wan Kim, Byung Joo Jung, Youngseok Lee, Eun Kyung Shim, Soyon Park, Eun Young Choi, Kyoo Sung Cho, Chang Sung Kim

Department of Periodontics

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Human bone marrow mesenchymal stem cells (hBMSCs) were isolated from bone marrow of the vertebral body. The hBMSCs were cultured under either hypoxic (1% O₂) or normoxic (21% O₂; control) conditions and the characteristics as mesenchymal stem cells were compared. Results revealed that hypoxia reduced proliferative potential and colony-forming efficiency of hBMSCs, and significantly enhanced osteogenic and chondrogenic differentiation. The hBMSCs enhanced the regenerative potential of bone in vivo. In vitro synthesis of soluble and insoluble collagen was significantly increased in the hypoxic condition. In vivo collagen tissue regeneration was also enhanced under the hypoxic condition, with concomitant increased expressions of various subtypes of collagen and lysyl-oxidase family mRNA. MicroRNA assays revealed that miR-155-5p, which negatively regulates HIF-1α, was significantly highly expressed. These observations demonstrate that hBMSCs obtained from human vertebrae exhibit altered characteristics under hypoxic conditions, and each factor contributing to hBMSC-mediated tissue healing should be evaluated with the goal of allowing their clinical application.

Original language	English
Pages (from-to)	34-45
Number of pages	12
Journal	Bone
Volume	78
DOIs	https://doi.org/10.1016/j.bone.2015.04.044
Publication status	Published - 2015 Sept 1

Bibliographical note

Funding Information:
This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MEST) (No. 2012M3A9C6049862 and No. 2012M3A9B2052521 ).

Publisher Copyright:
© 2015 Elsevier Inc.

All Science Journal Classification (ASJC) codes

Endocrinology, Diabetes and Metabolism
Physiology
Histology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bone.2015.04.044

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title = "Human bone marrow stem cells cultured under hypoxic conditions present altered characteristics and enhanced in vivo tissue regeneration",

abstract = "Human bone marrow mesenchymal stem cells (hBMSCs) were isolated from bone marrow of the vertebral body. The hBMSCs were cultured under either hypoxic (1% O2) or normoxic (21% O2; control) conditions and the characteristics as mesenchymal stem cells were compared. Results revealed that hypoxia reduced proliferative potential and colony-forming efficiency of hBMSCs, and significantly enhanced osteogenic and chondrogenic differentiation. The hBMSCs enhanced the regenerative potential of bone in vivo. In vitro synthesis of soluble and insoluble collagen was significantly increased in the hypoxic condition. In vivo collagen tissue regeneration was also enhanced under the hypoxic condition, with concomitant increased expressions of various subtypes of collagen and lysyl-oxidase family mRNA. MicroRNA assays revealed that miR-155-5p, which negatively regulates HIF-1α, was significantly highly expressed. These observations demonstrate that hBMSCs obtained from human vertebrae exhibit altered characteristics under hypoxic conditions, and each factor contributing to hBMSC-mediated tissue healing should be evaluated with the goal of allowing their clinical application.",

author = "Lee, {Jung Seok} and Park, {Jung Chul} and Kim, {Tae Wan} and Jung, {Byung Joo} and Youngseok Lee and Shim, {Eun Kyung} and Soyon Park and Choi, {Eun Young} and Cho, {Kyoo Sung} and Kim, {Chang Sung}",

note = "Funding Information: This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MEST) (No. 2012M3A9C6049862 and No. 2012M3A9B2052521 ). Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",

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doi = "10.1016/j.bone.2015.04.044",

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AU - Lee, Jung Seok

AU - Park, Jung Chul

AU - Kim, Tae Wan

AU - Jung, Byung Joo

AU - Lee, Youngseok

AU - Shim, Eun Kyung

AU - Park, Soyon

AU - Choi, Eun Young

AU - Cho, Kyoo Sung

AU - Kim, Chang Sung

N1 - Funding Information: This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MEST) (No. 2012M3A9C6049862 and No. 2012M3A9B2052521 ). Publisher Copyright: © 2015 Elsevier Inc.

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Human bone marrow mesenchymal stem cells (hBMSCs) were isolated from bone marrow of the vertebral body. The hBMSCs were cultured under either hypoxic (1% O2) or normoxic (21% O2; control) conditions and the characteristics as mesenchymal stem cells were compared. Results revealed that hypoxia reduced proliferative potential and colony-forming efficiency of hBMSCs, and significantly enhanced osteogenic and chondrogenic differentiation. The hBMSCs enhanced the regenerative potential of bone in vivo. In vitro synthesis of soluble and insoluble collagen was significantly increased in the hypoxic condition. In vivo collagen tissue regeneration was also enhanced under the hypoxic condition, with concomitant increased expressions of various subtypes of collagen and lysyl-oxidase family mRNA. MicroRNA assays revealed that miR-155-5p, which negatively regulates HIF-1α, was significantly highly expressed. These observations demonstrate that hBMSCs obtained from human vertebrae exhibit altered characteristics under hypoxic conditions, and each factor contributing to hBMSC-mediated tissue healing should be evaluated with the goal of allowing their clinical application.

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Human bone marrow stem cells cultured under hypoxic conditions present altered characteristics and enhanced in vivo tissue regeneration

Abstract

Bibliographical note

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UN SDGs

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