Hrp3, a chromodomain helicase/ATPase DNA binding protein, is required for heterochromatin silencing in fission yeast

Eung Jae Yoo; Yeun Kyu Jang; Myung Ae Lee; Pernilla Bjerling; Jae Bum Kim; Karl Ekwall; Rho Hyun Seong; Sang Dai Park

doi:10.1016/S0006-291X(02)00797-0

Hrp3, a chromodomain helicase/ATPase DNA binding protein, is required for heterochromatin silencing in fission yeast

Eung Jae Yoo, Yeun Kyu Jang, Myung Ae Lee, Pernilla Bjerling, Jae Bum Kim, Karl Ekwall, Rho Hyun Seong, Sang Dai Park

Department of Systems Biology

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Hrp3, a paralog of Hrp1, is a novel member of the CHD1 (chromo-helicase/ATPase-DNA binding 1) protein family of Schizosaccharomyces pombe. Although it has been considered that CHD1 proteins are required for chromatin modifications in transcriptional regulations, little is known about their roles in vivo. In this study, we examined the effects of Hrp3 on heterochromatin silencing using several S. pombe reporter strains. The phenotypic analysis revealed that hrp3⁺ is not an essential gene for cell viability. However, Hrp3 is required for transcriptional repression at silence loci of mat3. A chromatin immunoprecipitation assay showed that Hrp3 directly associates with mat3 chromatin. Thus, our results strongly suggest that Hrp3 is involved in heterochromatin silencing and plays a direct role as a chromatin remodeling factor at mat3 in vivo.

Original language	English
Pages (from-to)	970-974
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	295
Issue number	4
DOIs	https://doi.org/10.1016/S0006-291X(02)00797-0
Publication status	Published - 2002

Bibliographical note

Funding Information:
We thank R. Allshire and G. Thon for providing fission yeast strains, FY1497, FY121, and PG9 and J.A. Huberman for S. pombe rDNA. We also thank Dr. O. Hwang for the critical reading. This work was supported in part by a BK21 Research Fellowship from the Korea Ministry of Education and Korea Research Foundation Grant (R03-2001-00056). Work in the K.E. laboratory was supported by a grant from Cancerfonden #4284-B99.

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Hrp3, a chromodomain helicase/ATPase DNA binding protein, is required for heterochromatin silencing in fission yeast",

abstract = "Hrp3, a paralog of Hrp1, is a novel member of the CHD1 (chromo-helicase/ATPase-DNA binding 1) protein family of Schizosaccharomyces pombe. Although it has been considered that CHD1 proteins are required for chromatin modifications in transcriptional regulations, little is known about their roles in vivo. In this study, we examined the effects of Hrp3 on heterochromatin silencing using several S. pombe reporter strains. The phenotypic analysis revealed that hrp3+ is not an essential gene for cell viability. However, Hrp3 is required for transcriptional repression at silence loci of mat3. A chromatin immunoprecipitation assay showed that Hrp3 directly associates with mat3 chromatin. Thus, our results strongly suggest that Hrp3 is involved in heterochromatin silencing and plays a direct role as a chromatin remodeling factor at mat3 in vivo.",

author = "{Jae Yoo}, Eung and {Kyu Jang}, Yeun and {Ae Lee}, Myung and Pernilla Bjerling and {Bum Kim}, Jae and Karl Ekwall and {Hyun Seong}, Rho and {Dai Park}, Sang",

note = "Funding Information: We thank R. Allshire and G. Thon for providing fission yeast strains, FY1497, FY121, and PG9 and J.A. Huberman for S. pombe rDNA. We also thank Dr. O. Hwang for the critical reading. This work was supported in part by a BK21 Research Fellowship from the Korea Ministry of Education and Korea Research Foundation Grant (R03-2001-00056). Work in the K.E. laboratory was supported by a grant from Cancerfonden #4284-B99.",

year = "2002",

doi = "10.1016/S0006-291X(02)00797-0",

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pages = "970--974",

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T1 - Hrp3, a chromodomain helicase/ATPase DNA binding protein, is required for heterochromatin silencing in fission yeast

AU - Jae Yoo, Eung

AU - Kyu Jang, Yeun

AU - Ae Lee, Myung

AU - Bjerling, Pernilla

AU - Bum Kim, Jae

AU - Ekwall, Karl

AU - Hyun Seong, Rho

AU - Dai Park, Sang

N1 - Funding Information: We thank R. Allshire and G. Thon for providing fission yeast strains, FY1497, FY121, and PG9 and J.A. Huberman for S. pombe rDNA. We also thank Dr. O. Hwang for the critical reading. This work was supported in part by a BK21 Research Fellowship from the Korea Ministry of Education and Korea Research Foundation Grant (R03-2001-00056). Work in the K.E. laboratory was supported by a grant from Cancerfonden #4284-B99.

PY - 2002

Y1 - 2002

N2 - Hrp3, a paralog of Hrp1, is a novel member of the CHD1 (chromo-helicase/ATPase-DNA binding 1) protein family of Schizosaccharomyces pombe. Although it has been considered that CHD1 proteins are required for chromatin modifications in transcriptional regulations, little is known about their roles in vivo. In this study, we examined the effects of Hrp3 on heterochromatin silencing using several S. pombe reporter strains. The phenotypic analysis revealed that hrp3+ is not an essential gene for cell viability. However, Hrp3 is required for transcriptional repression at silence loci of mat3. A chromatin immunoprecipitation assay showed that Hrp3 directly associates with mat3 chromatin. Thus, our results strongly suggest that Hrp3 is involved in heterochromatin silencing and plays a direct role as a chromatin remodeling factor at mat3 in vivo.

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Hrp3, a chromodomain helicase/ATPase DNA binding protein, is required for heterochromatin silencing in fission yeast

Abstract

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