Histamine-induced Ca2+ influx via the PLA2/ lipoxygenase/TRPV1 pathway in rat sensory neurons

Byung Moon Kim, Sang Hee Lee, Won Sik Shim, Uhtaek Oh

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)


Histamine is known to excite a subset of C-fibers and cause itch sensation. Despite its well-defined excitatory action on sensory neurons, intracellular signaling mechanisms are not understood. Previously, we demonstrated that bradykinin excited sensory neurons by activating TRPV1 via the phospholipase A2 (PLA2) and lipoxygenase (LO) pathway. We, thus, hypothesized that histamine excited sensory neurons via the PLA 2/LO/TRPV1 pathway. Application of histamine elicited a rapid increase in intracellular Ca2+ ([Ca2+]i) that desensitized slowly in cultured dorsal root ganglion neurons. Histamine-induced [Ca2+]i was dependent on extracellular Ca2+ and inhibited by capsazepine and by SC0030, competitive antagonists of TRPV1. Quinacrine and nordihydroguaiaretic acid, a PLA2 and an LO inhibitor, respectively, blocked the histamine-induced Ca2+ influx in sensory neurons, while indomethacin (a cyclooxygenase inhibitor) did not. We thus conclude that histamine activates TRPV1 after stimulating the PLA2/LO pathway, leading to the excitation of sensory neurons. These results further provide an idea for potential use of TRPV1 antagonists as anti-itch drugs.

Original languageEnglish
Pages (from-to)159-162
Number of pages4
JournalNeuroscience Letters
Issue number1-3
Publication statusPublished - 2004 May 6

Bibliographical note

Funding Information:
This study was supported by the National Creative Research Initiatives of the Ministry of Science and Technology of Korea.

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)


Dive into the research topics of 'Histamine-induced Ca2+ influx via the PLA2/ lipoxygenase/TRPV1 pathway in rat sensory neurons'. Together they form a unique fingerprint.

Cite this