TY - JOUR
T1 - Highly selective electrochemical quantitation of creatinine based on its chemical reaction with 3,5-dinitrobenzoate
AU - Kim, Dong Hun
AU - Park, Jong Kwan
AU - Lee, Aejin
AU - Kim, Seok Cheol
AU - Chae, Ji Hee
AU - Lee, Minyoung
AU - Lee, Sang Guk
AU - Lee, Byung Wan
AU - Yun, Wan Soo
N1 - Publisher Copyright:
© 2023
PY - 2023/6/15
Y1 - 2023/6/15
N2 - A simple and reliable method for creatinine quantitation was developed for urine-based diagnosis as creatinine is an internal standard molecule for the calibration of fluctuating urinary concentration. Herein, the quantitation of creatinine in artificial urine was performed in the range of 1–30 mM, covering the normal concentration of human urine, using 3,5-dinitrobenzoate (3,5-DNB) as an electrochemical probe molecule. The chemical interference from various urinary species, including urea, uric acid, ammonia, ascorbic acid, alcohol, albumin, bilirubin, acetaminophen, and glucose, is confirmed to be significantly suppressed when the reaction of creatinine and 3,5-DNB is limited to 5 min with an increased OH– concentration. Additionally, the reliability of this method is confirmed for the use of real human urine samples by comparing the data obtained from this and with that of the conventional method.
AB - A simple and reliable method for creatinine quantitation was developed for urine-based diagnosis as creatinine is an internal standard molecule for the calibration of fluctuating urinary concentration. Herein, the quantitation of creatinine in artificial urine was performed in the range of 1–30 mM, covering the normal concentration of human urine, using 3,5-dinitrobenzoate (3,5-DNB) as an electrochemical probe molecule. The chemical interference from various urinary species, including urea, uric acid, ammonia, ascorbic acid, alcohol, albumin, bilirubin, acetaminophen, and glucose, is confirmed to be significantly suppressed when the reaction of creatinine and 3,5-DNB is limited to 5 min with an increased OH– concentration. Additionally, the reliability of this method is confirmed for the use of real human urine samples by comparing the data obtained from this and with that of the conventional method.
KW - 3,5-dinitrobenzoate
KW - Creatinine
KW - Electrochemical
KW - Quantitation
UR - http://www.scopus.com/inward/record.url?scp=85153575843&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85153575843&partnerID=8YFLogxK
U2 - 10.1016/j.jelechem.2023.117424
DO - 10.1016/j.jelechem.2023.117424
M3 - Article
AN - SCOPUS:85153575843
SN - 1572-6657
VL - 939
JO - Journal of Electroanalytical Chemistry
JF - Journal of Electroanalytical Chemistry
M1 - 117424
ER -