TY - GEN
T1 - High-throughput end-on imaging of drosophila embryo for quantitative analysis of morphogens and signaling
AU - Chung, Kwanghun
AU - Kim, Yoosik
AU - Gong, Emily
AU - Shvartsman, Stanislav
AU - Lu, Hang
PY - 2010
Y1 - 2010
N2 - Hydrodynamic forces due to the anisotropic shape of Drosophila embryo can rapidly orient it in an upright position. We used this effect and inertia hydrodynamics to design a microfluidics embryo trap array (META) that orders and vertically orients hundreds of embryos in a matter of minutes. Using META, we quantified morphogen gradients in the dorsoventral patterning system, a task that has been until now complicated by the need to manually orient individual embryos.
AB - Hydrodynamic forces due to the anisotropic shape of Drosophila embryo can rapidly orient it in an upright position. We used this effect and inertia hydrodynamics to design a microfluidics embryo trap array (META) that orders and vertically orients hundreds of embryos in a matter of minutes. Using META, we quantified morphogen gradients in the dorsoventral patterning system, a task that has been until now complicated by the need to manually orient individual embryos.
UR - http://www.scopus.com/inward/record.url?scp=84884321385&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884321385&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84884321385
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 1676
EP - 1678
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -