High-throughput detection of human salivary cortisol using a multiple optical probe based scanning system with micro-optics and nanograting coupled label-free microarray

Eikhyun Cho; Seoyeon Choi; Jongmyeong Shim; Taekyung Kim; Ryung Shin; Jinhyung Lee; Jongho Kim; Hyo Il Jung; Shinill Kang

doi:10.1016/j.snb.2016.04.076

High-throughput detection of human salivary cortisol using a multiple optical probe based scanning system with micro-optics and nanograting coupled label-free microarray

Eikhyun Cho, Seoyeon Choi, Jongmyeong Shim, Taekyung Kim, Ryung Shin, Jinhyung Lee, Jongho Kim, Hyo Il Jung, Shinill Kang

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We demonstrate the use of a parallel detection system with a nanograting-based microarray to accomplish high-throughput analysis of bio-molecular interactions in a label-free manner. Well-type label-free microarrays were fabricated to eliminate the risk of cross-contamination and to minimize sample volumes. Parallel analysis without the use of spectrometer arrays or a moving platform was accomplished by using scanning multiple optical probes generated by a spatial light modulator and microlens array. Additionally, multiple optical probe spots focused by the microlens array reduced detection errors while enhancing the signal-to-noise ratio within a high-density microarray. Finally, we verified the feasibility of the parallel detection system by analyzing the peak wavelength value (PWV) shift of human salivary cortisol and anti-cortisol in a competitive binding experiment.

Original language	English
Pages (from-to)	520-527
Number of pages	8
Journal	Sensors and Actuators, B: Chemical
Volume	233
DOIs	https://doi.org/10.1016/j.snb.2016.04.076
Publication status	Published - 2016 Oct 5

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea(NRF) Grant funded by the Korean Government(MSIP) (No. 2015R1A5A1037668 ) and in part by the Bio & Medical Technology Development Program of the NRF funded by the Korean government, MSIP (2015M3A9D7067364).

Publisher Copyright:
© 2016 Elsevier B.V. All rights reserved.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

Access to Document

10.1016/j.snb.2016.04.076

Cite this

@article{e1ecfa80f9b5496eaa22fd29b8e2a1fa,

title = "High-throughput detection of human salivary cortisol using a multiple optical probe based scanning system with micro-optics and nanograting coupled label-free microarray",

abstract = "We demonstrate the use of a parallel detection system with a nanograting-based microarray to accomplish high-throughput analysis of bio-molecular interactions in a label-free manner. Well-type label-free microarrays were fabricated to eliminate the risk of cross-contamination and to minimize sample volumes. Parallel analysis without the use of spectrometer arrays or a moving platform was accomplished by using scanning multiple optical probes generated by a spatial light modulator and microlens array. Additionally, multiple optical probe spots focused by the microlens array reduced detection errors while enhancing the signal-to-noise ratio within a high-density microarray. Finally, we verified the feasibility of the parallel detection system by analyzing the peak wavelength value (PWV) shift of human salivary cortisol and anti-cortisol in a competitive binding experiment.",

author = "Eikhyun Cho and Seoyeon Choi and Jongmyeong Shim and Taekyung Kim and Ryung Shin and Jinhyung Lee and Jongho Kim and Jung, {Hyo Il} and Shinill Kang",

note = "Funding Information: This work was supported by the National Research Foundation of Korea(NRF) Grant funded by the Korean Government(MSIP) (No. 2015R1A5A1037668 ) and in part by the Bio & Medical Technology Development Program of the NRF funded by the Korean government, MSIP (2015M3A9D7067364). Publisher Copyright: {\textcopyright} 2016 Elsevier B.V. All rights reserved.",

year = "2016",

month = oct,

day = "5",

doi = "10.1016/j.snb.2016.04.076",

language = "English",

volume = "233",

pages = "520--527",

journal = "Sensors and Actuators B: Chemical",

issn = "0925-4005",

publisher = "Elsevier",

}

TY - JOUR

T1 - High-throughput detection of human salivary cortisol using a multiple optical probe based scanning system with micro-optics and nanograting coupled label-free microarray

AU - Cho, Eikhyun

AU - Choi, Seoyeon

AU - Shim, Jongmyeong

AU - Kim, Taekyung

AU - Shin, Ryung

AU - Lee, Jinhyung

AU - Kim, Jongho

AU - Jung, Hyo Il

AU - Kang, Shinill

N1 - Funding Information: This work was supported by the National Research Foundation of Korea(NRF) Grant funded by the Korean Government(MSIP) (No. 2015R1A5A1037668 ) and in part by the Bio & Medical Technology Development Program of the NRF funded by the Korean government, MSIP (2015M3A9D7067364). Publisher Copyright: © 2016 Elsevier B.V. All rights reserved.

PY - 2016/10/5

Y1 - 2016/10/5

N2 - We demonstrate the use of a parallel detection system with a nanograting-based microarray to accomplish high-throughput analysis of bio-molecular interactions in a label-free manner. Well-type label-free microarrays were fabricated to eliminate the risk of cross-contamination and to minimize sample volumes. Parallel analysis without the use of spectrometer arrays or a moving platform was accomplished by using scanning multiple optical probes generated by a spatial light modulator and microlens array. Additionally, multiple optical probe spots focused by the microlens array reduced detection errors while enhancing the signal-to-noise ratio within a high-density microarray. Finally, we verified the feasibility of the parallel detection system by analyzing the peak wavelength value (PWV) shift of human salivary cortisol and anti-cortisol in a competitive binding experiment.

AB - We demonstrate the use of a parallel detection system with a nanograting-based microarray to accomplish high-throughput analysis of bio-molecular interactions in a label-free manner. Well-type label-free microarrays were fabricated to eliminate the risk of cross-contamination and to minimize sample volumes. Parallel analysis without the use of spectrometer arrays or a moving platform was accomplished by using scanning multiple optical probes generated by a spatial light modulator and microlens array. Additionally, multiple optical probe spots focused by the microlens array reduced detection errors while enhancing the signal-to-noise ratio within a high-density microarray. Finally, we verified the feasibility of the parallel detection system by analyzing the peak wavelength value (PWV) shift of human salivary cortisol and anti-cortisol in a competitive binding experiment.

UR - http://www.scopus.com/inward/record.url?scp=84964596031&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964596031&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2016.04.076

DO - 10.1016/j.snb.2016.04.076

M3 - Article

AN - SCOPUS:84964596031

SN - 0925-4005

VL - 233

SP - 520

EP - 527

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

ER -

High-throughput detection of human salivary cortisol using a multiple optical probe based scanning system with micro-optics and nanograting coupled label-free microarray

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this