High-throughput biomimetic 3D gel-island chip for investigating cancer cell heterogeneity

Zhixiong Zhang; Yu Chih Chen; Yi Luan; Euisik Yoon

doi:10.1109/MEMSYS.2016.7421720

High-throughput biomimetic 3D gel-island chip for investigating cancer cell heterogeneity

Zhixiong Zhang, Yu Chih Chen, Yi Luan, Euisik Yoon

Yonsei Advanced Science Institute

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Considerable evidence showing that ECM is an important regulator of cellular behavior including adhesion, metastasis, proliferation, and differentiation. Although hydrogel has been used in the cell culture, previous works focus on the collective cell behaviors rather than single cell heterogeneity. This paper reports a novel gel-islands microfluidic platform enabling single cells tracking in biomimetic three-dimensional (3D) microenvironment. The platform has the advantages of (1) reliable single cell encapsulation in 3D gel-islands, (2) high-throughput single-cell drug screening assays, and (3) automatic single cell lineage tracking. Combining gel-based 3D culture and single cell resolution, the presented platform can monitor cellular behaviors of hundreds of individual cells in 3D ECM microenvironment using small amount of matrix reagents and drugs.

Original language	English
Title of host publication	MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	691-694
Number of pages	4
ISBN (Electronic)	9781509019731
DOIs	https://doi.org/10.1109/MEMSYS.2016.7421720
Publication status	Published - 2016 Feb 26
Event	29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 - Shanghai, China Duration: 2016 Jan 24 → 2016 Jan 28

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2016-February
ISSN (Print)	1084-6999

Other

Other	29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
Country/Territory	China
City	Shanghai
Period	16/1/24 → 16/1/28

Bibliographical note

Publisher Copyright:
© 2016 IEEE.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/MEMSYS.2016.7421720

Cite this

Zhang, Z., Chen, Y. C., Luan, Y., & Yoon, E. (2016). High-throughput biomimetic 3D gel-island chip for investigating cancer cell heterogeneity. In MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems (pp. 691-694). Article 7421720 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2016-February). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2016.7421720

Zhang, Zhixiong ; Chen, Yu Chih ; Luan, Yi et al. / High-throughput biomimetic 3D gel-island chip for investigating cancer cell heterogeneity. MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2016. pp. 691-694 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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title = "High-throughput biomimetic 3D gel-island chip for investigating cancer cell heterogeneity",

abstract = "Considerable evidence showing that ECM is an important regulator of cellular behavior including adhesion, metastasis, proliferation, and differentiation. Although hydrogel has been used in the cell culture, previous works focus on the collective cell behaviors rather than single cell heterogeneity. This paper reports a novel gel-islands microfluidic platform enabling single cells tracking in biomimetic three-dimensional (3D) microenvironment. The platform has the advantages of (1) reliable single cell encapsulation in 3D gel-islands, (2) high-throughput single-cell drug screening assays, and (3) automatic single cell lineage tracking. Combining gel-based 3D culture and single cell resolution, the presented platform can monitor cellular behaviors of hundreds of individual cells in 3D ECM microenvironment using small amount of matrix reagents and drugs.",

author = "Zhixiong Zhang and Chen, {Yu Chih} and Yi Luan and Euisik Yoon",

note = "Publisher Copyright: {\textcopyright} 2016 IEEE.; 29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 ; Conference date: 24-01-2016 Through 28-01-2016",

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language = "English",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

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Zhang, Z, Chen, YC, Luan, Y & Yoon, E 2016, High-throughput biomimetic 3D gel-island chip for investigating cancer cell heterogeneity. in MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems., 7421720, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2016-February, Institute of Electrical and Electronics Engineers Inc., pp. 691-694, 29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016, Shanghai, China, 16/1/24. https://doi.org/10.1109/MEMSYS.2016.7421720

High-throughput biomimetic 3D gel-island chip for investigating cancer cell heterogeneity. / Zhang, Zhixiong; Chen, Yu Chih; Luan, Yi et al.
MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2016. p. 691-694 7421720 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2016-February).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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PY - 2016/2/26

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N2 - Considerable evidence showing that ECM is an important regulator of cellular behavior including adhesion, metastasis, proliferation, and differentiation. Although hydrogel has been used in the cell culture, previous works focus on the collective cell behaviors rather than single cell heterogeneity. This paper reports a novel gel-islands microfluidic platform enabling single cells tracking in biomimetic three-dimensional (3D) microenvironment. The platform has the advantages of (1) reliable single cell encapsulation in 3D gel-islands, (2) high-throughput single-cell drug screening assays, and (3) automatic single cell lineage tracking. Combining gel-based 3D culture and single cell resolution, the presented platform can monitor cellular behaviors of hundreds of individual cells in 3D ECM microenvironment using small amount of matrix reagents and drugs.

AB - Considerable evidence showing that ECM is an important regulator of cellular behavior including adhesion, metastasis, proliferation, and differentiation. Although hydrogel has been used in the cell culture, previous works focus on the collective cell behaviors rather than single cell heterogeneity. This paper reports a novel gel-islands microfluidic platform enabling single cells tracking in biomimetic three-dimensional (3D) microenvironment. The platform has the advantages of (1) reliable single cell encapsulation in 3D gel-islands, (2) high-throughput single-cell drug screening assays, and (3) automatic single cell lineage tracking. Combining gel-based 3D culture and single cell resolution, the presented platform can monitor cellular behaviors of hundreds of individual cells in 3D ECM microenvironment using small amount of matrix reagents and drugs.

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Zhang Z, Chen YC, Luan Y, Yoon E. High-throughput biomimetic 3D gel-island chip for investigating cancer cell heterogeneity. In MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc. 2016. p. 691-694. 7421720. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2016.7421720

High-throughput biomimetic 3D gel-island chip for investigating cancer cell heterogeneity

Abstract

Publication series

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Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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Cite this