The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery. Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function. Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels. Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors. The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue. Our study can be referenced to fabricate various types of artificial tissues that mimic the original functions as well as structures.
Bibliographical noteFunding Information:
This work was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea Government (2015R1A2A2A01007602, 2017R1A2B3005994, 2018R1A2A3075287), the Institute of Convergence Science (ICONS) at Yonsei University, the Institute for Basic Science (IBS-R026-D1), Global Research Cooperation Funding provided by Emory University and Yonsei University, and Brain Korea 21 Plus Project (Mechanical Technology Global Leader Program for Society Development, Initiative for Biological Function & Systems) in 2018.
© 2018, The Author(s).
All Science Journal Classification (ASJC) codes
- Physics and Astronomy(all)
- Biochemistry, Genetics and Molecular Biology(all)