High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy

Byungjun Kang, Jisoo Shin, Hyun Ji Park, Chanryeol Rhyou, Donyoung Kang, Shin Jeong Lee, Young sup Yoon, Seung Woo Cho, Hyungsuk Lee

Research output: Contribution to journalArticlepeer-review

92 Citations (Scopus)


The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery. Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function. Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels. Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors. The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue. Our study can be referenced to fabricate various types of artificial tissues that mimic the original functions as well as structures.

Original languageEnglish
Article number5402
JournalNature communications
Issue number1
Publication statusPublished - 2018 Dec 1

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea Government (2015R1A2A2A01007602, 2017R1A2B3005994, 2018R1A2A3075287), the Institute of Convergence Science (ICONS) at Yonsei University, the Institute for Basic Science (IBS-R026-D1), Global Research Cooperation Funding provided by Emory University and Yonsei University, and Brain Korea 21 Plus Project (Mechanical Technology Global Leader Program for Society Development, Initiative for Biological Function & Systems) in 2018.

Publisher Copyright:
© 2018, The Author(s).

All Science Journal Classification (ASJC) codes

  • General
  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)


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