TY - GEN
T1 - High-density array of single cell traps for high-throughput imaging of calcium dynamics in response to oxidative stress
AU - Rivet, Catherine A.
AU - Chung, Kwanghun
AU - Kemp, Melissa L.
AU - Lu, Hang
PY - 2010
Y1 - 2010
N2 - Stochasticity in gene expression, protein or metabolite levels contributes to cell-cell variations. The analysis of these variations could lead to a better understanding of cellular processes and drug responses, but require single cell analysis techniques. To this end, we developed a new microfluidic platform for high-throughput capture (in about a minute) with low shear and imaging of thousands of single cells. This platform is compatible with upstream components for complex cell stimulation patterns. Imaging of calcium dynamics in response to oxidative stress with this platform reveals variable phenotypic responses within clonal populations.
AB - Stochasticity in gene expression, protein or metabolite levels contributes to cell-cell variations. The analysis of these variations could lead to a better understanding of cellular processes and drug responses, but require single cell analysis techniques. To this end, we developed a new microfluidic platform for high-throughput capture (in about a minute) with low shear and imaging of thousands of single cells. This platform is compatible with upstream components for complex cell stimulation patterns. Imaging of calcium dynamics in response to oxidative stress with this platform reveals variable phenotypic responses within clonal populations.
UR - http://www.scopus.com/inward/record.url?scp=84884331810&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884331810&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84884331810
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 2041
EP - 2043
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -