Hemoglobin-mimetic oxygen adsorbent prepared via self-assembly of cysteinyl bolaamphiphiles

Chaemyeong Lee; Min Chul Kim; Sang Yup Lee

doi:10.1016/j.colsurfb.2016.03.012

Hemoglobin-mimetic oxygen adsorbent prepared via self-assembly of cysteinyl bolaamphiphiles

Chaemyeong Lee, Min Chul Kim, Sang Yup Lee

Department of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

In this study, a novel cysteinyl bolaamphiphilic molecule was synthesized and its self-assembled planar suprastructure was applied as a biomimetic matrix to create a hemoglobin-mimetic oxygen adsorbent that exploits the ability of cysteine thiols to bind hemin. Self-assembly of the cysteinyl bolaamphiphilic molecule exposed cysteine thiols on its surface in the presence of β-mercaptoethanol, known to reduce disulfide bonds, without which, helically coiled structures were generated. The self-assembled planar structure was used as a soft matrix to create a hemoglobin-mimetic oxygen adsorbent. The surface-exposed cysteine thiols were used to attach hemin, producing a hemin-bound, planar structure mimicking hemoglobin. This hemoglobin mimic strongly adsorbed oxygen and remained stable up to 50 °C. The cysteinyl bolaamphiphile self-assembled structure provided a biomimetic platform that allowed for the association of biological substances in a manner similar to natural proteins.

Original language	English
Pages (from-to)	360-366
Number of pages	7
Journal	Colloids and Surfaces B: Biointerfaces
Volume	142
DOIs	https://doi.org/10.1016/j.colsurfb.2016.03.012
Publication status	Published - 2016 Jun 1

Bibliographical note

Funding Information:
This work was supported by a grant from the Korean Research Foundation funded by the Korean Government ( NRF-2013R1A1A2006459 ) and by the Human Resources Program in Energy Technology of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was provided with financial resources from the Ministry of Trade, Industry & Energy, Republic of Korea (No. 20154010200810 ).

Publisher Copyright:
© 2016 Elsevier B.V.

All Science Journal Classification (ASJC) codes

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

Access to Document

10.1016/j.colsurfb.2016.03.012

Cite this

@article{5b39446462e84a2d86864f18a95d083c,

title = "Hemoglobin-mimetic oxygen adsorbent prepared via self-assembly of cysteinyl bolaamphiphiles",

abstract = "In this study, a novel cysteinyl bolaamphiphilic molecule was synthesized and its self-assembled planar suprastructure was applied as a biomimetic matrix to create a hemoglobin-mimetic oxygen adsorbent that exploits the ability of cysteine thiols to bind hemin. Self-assembly of the cysteinyl bolaamphiphilic molecule exposed cysteine thiols on its surface in the presence of β-mercaptoethanol, known to reduce disulfide bonds, without which, helically coiled structures were generated. The self-assembled planar structure was used as a soft matrix to create a hemoglobin-mimetic oxygen adsorbent. The surface-exposed cysteine thiols were used to attach hemin, producing a hemin-bound, planar structure mimicking hemoglobin. This hemoglobin mimic strongly adsorbed oxygen and remained stable up to 50 °C. The cysteinyl bolaamphiphile self-assembled structure provided a biomimetic platform that allowed for the association of biological substances in a manner similar to natural proteins.",

author = "Chaemyeong Lee and Kim, {Min Chul} and Lee, {Sang Yup}",

note = "Funding Information: This work was supported by a grant from the Korean Research Foundation funded by the Korean Government ( NRF-2013R1A1A2006459 ) and by the Human Resources Program in Energy Technology of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was provided with financial resources from the Ministry of Trade, Industry & Energy, Republic of Korea (No. 20154010200810 ). Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2016",

month = jun,

day = "1",

doi = "10.1016/j.colsurfb.2016.03.012",

language = "English",

volume = "142",

pages = "360--366",

journal = "Colloids and Surfaces B: Biointerfaces",

issn = "0927-7765",

publisher = "Elsevier",

}

TY - JOUR

T1 - Hemoglobin-mimetic oxygen adsorbent prepared via self-assembly of cysteinyl bolaamphiphiles

AU - Lee, Chaemyeong

AU - Kim, Min Chul

AU - Lee, Sang Yup

N1 - Funding Information: This work was supported by a grant from the Korean Research Foundation funded by the Korean Government ( NRF-2013R1A1A2006459 ) and by the Human Resources Program in Energy Technology of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was provided with financial resources from the Ministry of Trade, Industry & Energy, Republic of Korea (No. 20154010200810 ). Publisher Copyright: © 2016 Elsevier B.V.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - In this study, a novel cysteinyl bolaamphiphilic molecule was synthesized and its self-assembled planar suprastructure was applied as a biomimetic matrix to create a hemoglobin-mimetic oxygen adsorbent that exploits the ability of cysteine thiols to bind hemin. Self-assembly of the cysteinyl bolaamphiphilic molecule exposed cysteine thiols on its surface in the presence of β-mercaptoethanol, known to reduce disulfide bonds, without which, helically coiled structures were generated. The self-assembled planar structure was used as a soft matrix to create a hemoglobin-mimetic oxygen adsorbent. The surface-exposed cysteine thiols were used to attach hemin, producing a hemin-bound, planar structure mimicking hemoglobin. This hemoglobin mimic strongly adsorbed oxygen and remained stable up to 50 °C. The cysteinyl bolaamphiphile self-assembled structure provided a biomimetic platform that allowed for the association of biological substances in a manner similar to natural proteins.

AB - In this study, a novel cysteinyl bolaamphiphilic molecule was synthesized and its self-assembled planar suprastructure was applied as a biomimetic matrix to create a hemoglobin-mimetic oxygen adsorbent that exploits the ability of cysteine thiols to bind hemin. Self-assembly of the cysteinyl bolaamphiphilic molecule exposed cysteine thiols on its surface in the presence of β-mercaptoethanol, known to reduce disulfide bonds, without which, helically coiled structures were generated. The self-assembled planar structure was used as a soft matrix to create a hemoglobin-mimetic oxygen adsorbent. The surface-exposed cysteine thiols were used to attach hemin, producing a hemin-bound, planar structure mimicking hemoglobin. This hemoglobin mimic strongly adsorbed oxygen and remained stable up to 50 °C. The cysteinyl bolaamphiphile self-assembled structure provided a biomimetic platform that allowed for the association of biological substances in a manner similar to natural proteins.

UR - http://www.scopus.com/inward/record.url?scp=84960415517&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84960415517&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2016.03.012

DO - 10.1016/j.colsurfb.2016.03.012

M3 - Article

C2 - 26970824

AN - SCOPUS:84960415517

SN - 0927-7765

VL - 142

SP - 360

EP - 366

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

ER -

Hemoglobin-mimetic oxygen adsorbent prepared via self-assembly of cysteinyl bolaamphiphiles

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this