Global and focused transcriptional profiling of small molecule aminopeptidase N inhibitor reveals its mechanism of angiogenesis inhibition

Joong Sup Shim; Hyo Mi Park; Jiyong Lee; Ho Jeong Kwon

doi:10.1016/j.bbrc.2008.04.023

Global and focused transcriptional profiling of small molecule aminopeptidase N inhibitor reveals its mechanism of angiogenesis inhibition

Joong Sup Shim, Hyo Mi Park, Jiyong Lee, Ho Jeong Kwon

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We recently developed a specific small molecule inhibitor of aminopeptidase N (APN), named as HNSA, through a high throughput screening. In the present study, we investigated the major cellular phenotypes of HNSA in comparison with those of APN knock-down in human fibrosarcoma cells and the mechanism of angiogenesis inhibition by the compound using DNA microarray analyses. Global gene expression analyses showed that HNSA signatures are significantly correlated with those of APN knock-down in HT1080 cells, suggesting that APN is a primary target of HNSA in the cells. Using the angiogenesis-focused DNA microarrays, nine of angiogenesis-related genes were identified as crucial mediators of angiogenesis inhibition by HNSA. These data demonstrate that HNSA can be used as a valuable tool to decipher the APN function in angiogenesis.

Original language	English
Pages (from-to)	99-103
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	371
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2008.04.023
Publication status	Published - 2008 Jun 20

Bibliographical note

Funding Information:
This research was supported by a Grant (07142KFDA696) from Korea Food & Drug Administration and from the Brain Korea 21 Project, Republic of Korea.

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.04.023

Cite this

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title = "Global and focused transcriptional profiling of small molecule aminopeptidase N inhibitor reveals its mechanism of angiogenesis inhibition",

abstract = "We recently developed a specific small molecule inhibitor of aminopeptidase N (APN), named as HNSA, through a high throughput screening. In the present study, we investigated the major cellular phenotypes of HNSA in comparison with those of APN knock-down in human fibrosarcoma cells and the mechanism of angiogenesis inhibition by the compound using DNA microarray analyses. Global gene expression analyses showed that HNSA signatures are significantly correlated with those of APN knock-down in HT1080 cells, suggesting that APN is a primary target of HNSA in the cells. Using the angiogenesis-focused DNA microarrays, nine of angiogenesis-related genes were identified as crucial mediators of angiogenesis inhibition by HNSA. These data demonstrate that HNSA can be used as a valuable tool to decipher the APN function in angiogenesis.",

author = "Shim, {Joong Sup} and Park, {Hyo Mi} and Jiyong Lee and Kwon, {Ho Jeong}",

note = "Funding Information: This research was supported by a Grant (07142KFDA696) from Korea Food & Drug Administration and from the Brain Korea 21 Project, Republic of Korea.",

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doi = "10.1016/j.bbrc.2008.04.023",

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AU - Shim, Joong Sup

AU - Park, Hyo Mi

AU - Lee, Jiyong

AU - Kwon, Ho Jeong

N1 - Funding Information: This research was supported by a Grant (07142KFDA696) from Korea Food & Drug Administration and from the Brain Korea 21 Project, Republic of Korea.

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Global and focused transcriptional profiling of small molecule aminopeptidase N inhibitor reveals its mechanism of angiogenesis inhibition

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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