Gentisyl alcohol inhibits apoptosis by suppressing caspase activity induced by etoposide

Jinhee Kim; Donghyun Kim; Meeree Kim; Hojeong Kwon; Taekwang Oh; Choonghwan Lee

Gentisyl alcohol inhibits apoptosis by suppressing caspase activity induced by etoposide

Jinhee Kim, Donghyun Kim, Meeree Kim, Hojeong Kwon, Taekwang Oh, Choonghwan Lee

Research output: Contribution to journal › Article › peer-review

Abstract

In the course of our screening for small molecules to inhibit apoptosis of U937 human leukemia cells induced by etoposide (10 μg/ml), Penicillium sp. F020150 with potent inhibitory activity was selected. The active compound was purified from ethyl acetate extract of the microorganism by Sephadex LH-20 column chromatography and HPLC, and was identified as gentisyl alcohol (2,5-dihydroxybenzyl alcohol) by spectroscopic methods. The compound inhibited caspase-3 induction with IC₅₀ value of 3.0 μg/ml after 8 h of etoposide treatment. The expression levels of caspase-3 and PARP were dose-dependently inhibited by the compound, suggesting that gentisyl alcohol inhibits etoposide-induced apoptosis via downregulation of caspases.

Original language	English
Pages (from-to)	532-536
Number of pages	5
Journal	Journal of microbiology and biotechnology
Volume	15
Issue number	3
Publication status	Published - 2005 Jun

All Science Journal Classification (ASJC) codes

Biotechnology
Applied Microbiology and Biotechnology

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title = "Gentisyl alcohol inhibits apoptosis by suppressing caspase activity induced by etoposide",

abstract = "In the course of our screening for small molecules to inhibit apoptosis of U937 human leukemia cells induced by etoposide (10 μg/ml), Penicillium sp. F020150 with potent inhibitory activity was selected. The active compound was purified from ethyl acetate extract of the microorganism by Sephadex LH-20 column chromatography and HPLC, and was identified as gentisyl alcohol (2,5-dihydroxybenzyl alcohol) by spectroscopic methods. The compound inhibited caspase-3 induction with IC50 value of 3.0 μg/ml after 8 h of etoposide treatment. The expression levels of caspase-3 and PARP were dose-dependently inhibited by the compound, suggesting that gentisyl alcohol inhibits etoposide-induced apoptosis via downregulation of caspases.",

author = "Jinhee Kim and Donghyun Kim and Meeree Kim and Hojeong Kwon and Taekwang Oh and Choonghwan Lee",

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T1 - Gentisyl alcohol inhibits apoptosis by suppressing caspase activity induced by etoposide

AU - Kim, Jinhee

AU - Kim, Donghyun

AU - Kim, Meeree

AU - Kwon, Hojeong

AU - Oh, Taekwang

AU - Lee, Choonghwan

PY - 2005/6

Y1 - 2005/6

N2 - In the course of our screening for small molecules to inhibit apoptosis of U937 human leukemia cells induced by etoposide (10 μg/ml), Penicillium sp. F020150 with potent inhibitory activity was selected. The active compound was purified from ethyl acetate extract of the microorganism by Sephadex LH-20 column chromatography and HPLC, and was identified as gentisyl alcohol (2,5-dihydroxybenzyl alcohol) by spectroscopic methods. The compound inhibited caspase-3 induction with IC50 value of 3.0 μg/ml after 8 h of etoposide treatment. The expression levels of caspase-3 and PARP were dose-dependently inhibited by the compound, suggesting that gentisyl alcohol inhibits etoposide-induced apoptosis via downregulation of caspases.

AB - In the course of our screening for small molecules to inhibit apoptosis of U937 human leukemia cells induced by etoposide (10 μg/ml), Penicillium sp. F020150 with potent inhibitory activity was selected. The active compound was purified from ethyl acetate extract of the microorganism by Sephadex LH-20 column chromatography and HPLC, and was identified as gentisyl alcohol (2,5-dihydroxybenzyl alcohol) by spectroscopic methods. The compound inhibited caspase-3 induction with IC50 value of 3.0 μg/ml after 8 h of etoposide treatment. The expression levels of caspase-3 and PARP were dose-dependently inhibited by the compound, suggesting that gentisyl alcohol inhibits etoposide-induced apoptosis via downregulation of caspases.

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Gentisyl alcohol inhibits apoptosis by suppressing caspase activity induced by etoposide

Abstract

All Science Journal Classification (ASJC) codes

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