Genotypes and genetic characters of Mycobacterium tuberculosis from Myanmar using three typing methods

Phyu Win Ei, Jong Seok Lee, Wah Wah Aung, Kim Yeun, Workneh Korma, Wint Wint Nyunt, Thyn Lei Swe, Mi Mi Htwe, Su Mon Win, Si Thu Aung, Chulhun L. Chang, Hyeyoung Lee

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Knowledge on basic characteristics of Mycobacterium tuberculosis (MTB) is helpful to understand the disease epidemiology and support the prediction of clinical outcome of the disease. The aim of this study was to detect the genotypes and genotypic characters of clinical Mycobacterium tuberculosis (MTB) isolates from new and retreatment rifampicin-resistant patients using three different genotyping methods. Mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) typing was used to determine the diversity of 222 clinical isolates. Spoligotyping and IS6110-restriction fragment length polymorphism (RFLP) typing were also used to investigate the genetic characters of 105 MTB strains. Among the 15 genotypes detected by MIRU-VNTR, Beijing strains were the most prevalent of all strains (54.8%); new cases (40.5%) and retreatment cases (69.4%), followed by EAI strain. Spoligotyping categorized the strains into 11 lineages and 13 orphans whereas 96 different IS6110 patterns were identified using RFLP method. The mode number of IS6110 was 18 and 20. Higher band numbers were found in Beijing genotype (p < 0.001). Clustering rates by spoligotyping, MIRU-VNTR and IS6110-RFLP typing were 0.714, 0.004 and 0.085, respectively. Discriminatory powers of spoligotyping, MIRU-VNTR typing and IS6110-RFLP typing were 0.637, 1.000 and 0.997, respectively. Dominant Beijing genotype in both new and retreatment cases denoting that prevailing tuberculosis in Myanmar changed from EAI to Beijing lineage.

Original languageEnglish
Article number104005
JournalInfection, Genetics and Evolution
Publication statusPublished - 2019 Nov

Bibliographical note

Publisher Copyright:
© 2019

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases


Dive into the research topics of 'Genotypes and genetic characters of Mycobacterium tuberculosis from Myanmar using three typing methods'. Together they form a unique fingerprint.

Cite this