Genetic Manipulation of Cryptococcus neoformans

Kwang Woo Jung; Kyung Tae Lee; Yee Seul So; Yong Sun Bahn

doi:10.1002/cpmc.59

Genetic Manipulation of Cryptococcus neoformans

Kwang Woo Jung, Kyung Tae Lee, Yee Seul So, Yong Sun Bahn

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Cryptococcus neoformans is an opportunistic fungal pathogen, which causes life-threatening meningoencephalitis in immunocompromised individuals and is responsible for more than 1,000,000 infections and 600,000 deaths annually worldwide. Nevertheless, anti-cryptococcal therapeutic options are limited, mainly because of the similarity between fungal and human cellular structures. Owing to advances in genetic and molecular techniques and bioinformatics in the past decade, C. neoformans, belonging to the phylum basidiomycota, is now a major pathogenic fungal model system. In particular, genetic manipulation is the first step in the identification and characterization of the function of genes for understanding the mechanisms underlying the pathogenicity of C. neoformans. This unit describes protocols for constructing target gene deletion mutants using double-joint (DJ) PCR, constitutive overexpression strains using the histone H3 gene promoter, and epitope/fluorescence protein-tagged strains in C. neoformans.

Original language	English
Article number	e59
Journal	Current Protocols in Microbiology
Volume	50
Issue number	1
DOIs	https://doi.org/10.1002/cpmc.59
Publication status	Published - 2018 Aug

Bibliographical note

Funding Information:
This work was supported by National Research Foundation of Korea grants (2016R1E1A1A01943365) from Ministry of Science and ICT. The authors declare that they have no conflicts of interest.

Funding Information:
This work was supported by Na tional Research Foundation of Korea grants (2016R1E1A1A01943365) from Ministry of Science and ICT. The authors declare that they have no conflicts of interest.

Publisher Copyright:
© 2018 John Wiley & Sons, Inc.

All Science Journal Classification (ASJC) codes

Parasitology
Microbiology
Virology

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10.1002/cpmc.59

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title = "Genetic Manipulation of Cryptococcus neoformans",

abstract = "Cryptococcus neoformans is an opportunistic fungal pathogen, which causes life-threatening meningoencephalitis in immunocompromised individuals and is responsible for more than 1,000,000 infections and 600,000 deaths annually worldwide. Nevertheless, anti-cryptococcal therapeutic options are limited, mainly because of the similarity between fungal and human cellular structures. Owing to advances in genetic and molecular techniques and bioinformatics in the past decade, C. neoformans, belonging to the phylum basidiomycota, is now a major pathogenic fungal model system. In particular, genetic manipulation is the first step in the identification and characterization of the function of genes for understanding the mechanisms underlying the pathogenicity of C. neoformans. This unit describes protocols for constructing target gene deletion mutants using double-joint (DJ) PCR, constitutive overexpression strains using the histone H3 gene promoter, and epitope/fluorescence protein-tagged strains in C. neoformans.",

author = "Jung, {Kwang Woo} and Lee, {Kyung Tae} and So, {Yee Seul} and Bahn, {Yong Sun}",

note = "Funding Information: This work was supported by National Research Foundation of Korea grants (2016R1E1A1A01943365) from Ministry of Science and ICT. The authors declare that they have no conflicts of interest. Funding Information: This work was supported by Na tional Research Foundation of Korea grants (2016R1E1A1A01943365) from Ministry of Science and ICT. The authors declare that they have no conflicts of interest. Publisher Copyright: {\textcopyright} 2018 John Wiley & Sons, Inc.",

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AU - Bahn, Yong Sun

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N2 - Cryptococcus neoformans is an opportunistic fungal pathogen, which causes life-threatening meningoencephalitis in immunocompromised individuals and is responsible for more than 1,000,000 infections and 600,000 deaths annually worldwide. Nevertheless, anti-cryptococcal therapeutic options are limited, mainly because of the similarity between fungal and human cellular structures. Owing to advances in genetic and molecular techniques and bioinformatics in the past decade, C. neoformans, belonging to the phylum basidiomycota, is now a major pathogenic fungal model system. In particular, genetic manipulation is the first step in the identification and characterization of the function of genes for understanding the mechanisms underlying the pathogenicity of C. neoformans. This unit describes protocols for constructing target gene deletion mutants using double-joint (DJ) PCR, constitutive overexpression strains using the histone H3 gene promoter, and epitope/fluorescence protein-tagged strains in C. neoformans.

AB - Cryptococcus neoformans is an opportunistic fungal pathogen, which causes life-threatening meningoencephalitis in immunocompromised individuals and is responsible for more than 1,000,000 infections and 600,000 deaths annually worldwide. Nevertheless, anti-cryptococcal therapeutic options are limited, mainly because of the similarity between fungal and human cellular structures. Owing to advances in genetic and molecular techniques and bioinformatics in the past decade, C. neoformans, belonging to the phylum basidiomycota, is now a major pathogenic fungal model system. In particular, genetic manipulation is the first step in the identification and characterization of the function of genes for understanding the mechanisms underlying the pathogenicity of C. neoformans. This unit describes protocols for constructing target gene deletion mutants using double-joint (DJ) PCR, constitutive overexpression strains using the histone H3 gene promoter, and epitope/fluorescence protein-tagged strains in C. neoformans.

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Genetic Manipulation of Cryptococcus neoformans

Abstract

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