Genetic and biochemical characterization of GES-5, an extended-spectrum class A β-lactamase from Klebsiella pneumoniae

Il Kwon Bae; You Nae Lee; Seok Hoon Jeong; Seong Geun Hong; Jung Hun Lee; Sang Hee Lee; Hyoung Jin Kim; Hasik Youn

doi:10.1016/j.diagmicrobio.2007.02.013

Genetic and biochemical characterization of GES-5, an extended-spectrum class A β-lactamase from Klebsiella pneumoniae

Il Kwon Bae, You Nae Lee, Seok Hoon Jeong, Seong Geun Hong, Jung Hun Lee, Sang Hee Lee, Hyoung Jin Kim, Hasik Youn

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

The present study was conducted to characterize a new class 1 integron containing the bla_GES-5 gene cassette in Klebsiella pneumoniae clinical isolate CHAK36 and measure the kinetic parameters of GES-5 β-lactamase. Long-range polymerase chain reactions (PCRs) and sequence analysis were performed to identify and analyze the bla_GES-5 gene cassette-containing integrons. Kinetic parameters were determined from purified GES-5. Sequencing of the 6190-bp PCR amplicon from K. pneumoniae CHAK36 isolate revealed the new structure of class 1 integron. The integron has 3 unique gene cassettes (bla_GES-5-aac(6′)-IIa-bla_OXA-17/orf4), but the 59-base element of the bla_OXA-17 gene cassette was interrupted by a putative transposase gene, orf4. The kinetic parameters of GES-5 showed its broad-spectrum activity against most β-lactams, including benzylpenicillin, cefaloridine, cefotaxime, and imipenem. This work shows that the bla_GES-5 gene was located on a new class 1 integron as a gene cassette. Our kinetic characterizations show that GES-5 was more active against impenem than GES-2 and GES-4.

Original language	English
Pages (from-to)	465-468
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	58
Issue number	4
DOIs	https://doi.org/10.1016/j.diagmicrobio.2007.02.013
Publication status	Published - 2007 Aug

Bibliographical note

Funding Information:
This work was supported by the Korea Research Foundation grant (KRF-2006-331-E00455).

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.diagmicrobio.2007.02.013

Cite this

@article{b133bcf47a534a45847e41caeebba7ae,

title = "Genetic and biochemical characterization of GES-5, an extended-spectrum class A β-lactamase from Klebsiella pneumoniae",

abstract = "The present study was conducted to characterize a new class 1 integron containing the blaGES-5 gene cassette in Klebsiella pneumoniae clinical isolate CHAK36 and measure the kinetic parameters of GES-5 β-lactamase. Long-range polymerase chain reactions (PCRs) and sequence analysis were performed to identify and analyze the blaGES-5 gene cassette-containing integrons. Kinetic parameters were determined from purified GES-5. Sequencing of the 6190-bp PCR amplicon from K. pneumoniae CHAK36 isolate revealed the new structure of class 1 integron. The integron has 3 unique gene cassettes (blaGES-5-aac(6′)-IIa-blaOXA-17/orf4), but the 59-base element of the blaOXA-17 gene cassette was interrupted by a putative transposase gene, orf4. The kinetic parameters of GES-5 showed its broad-spectrum activity against most β-lactams, including benzylpenicillin, cefaloridine, cefotaxime, and imipenem. This work shows that the blaGES-5 gene was located on a new class 1 integron as a gene cassette. Our kinetic characterizations show that GES-5 was more active against impenem than GES-2 and GES-4.",

author = "Bae, {Il Kwon} and Lee, {You Nae} and Jeong, {Seok Hoon} and Hong, {Seong Geun} and Lee, {Jung Hun} and Lee, {Sang Hee} and Kim, {Hyoung Jin} and Hasik Youn",

note = "Funding Information: This work was supported by the Korea Research Foundation grant (KRF-2006-331-E00455).",

year = "2007",

month = aug,

doi = "10.1016/j.diagmicrobio.2007.02.013",

language = "English",

volume = "58",

pages = "465--468",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier Inc.",

number = "4",

}

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T1 - Genetic and biochemical characterization of GES-5, an extended-spectrum class A β-lactamase from Klebsiella pneumoniae

AU - Bae, Il Kwon

AU - Lee, You Nae

AU - Jeong, Seok Hoon

AU - Hong, Seong Geun

AU - Lee, Jung Hun

AU - Lee, Sang Hee

AU - Kim, Hyoung Jin

AU - Youn, Hasik

N1 - Funding Information: This work was supported by the Korea Research Foundation grant (KRF-2006-331-E00455).

PY - 2007/8

Y1 - 2007/8

N2 - The present study was conducted to characterize a new class 1 integron containing the blaGES-5 gene cassette in Klebsiella pneumoniae clinical isolate CHAK36 and measure the kinetic parameters of GES-5 β-lactamase. Long-range polymerase chain reactions (PCRs) and sequence analysis were performed to identify and analyze the blaGES-5 gene cassette-containing integrons. Kinetic parameters were determined from purified GES-5. Sequencing of the 6190-bp PCR amplicon from K. pneumoniae CHAK36 isolate revealed the new structure of class 1 integron. The integron has 3 unique gene cassettes (blaGES-5-aac(6′)-IIa-blaOXA-17/orf4), but the 59-base element of the blaOXA-17 gene cassette was interrupted by a putative transposase gene, orf4. The kinetic parameters of GES-5 showed its broad-spectrum activity against most β-lactams, including benzylpenicillin, cefaloridine, cefotaxime, and imipenem. This work shows that the blaGES-5 gene was located on a new class 1 integron as a gene cassette. Our kinetic characterizations show that GES-5 was more active against impenem than GES-2 and GES-4.

AB - The present study was conducted to characterize a new class 1 integron containing the blaGES-5 gene cassette in Klebsiella pneumoniae clinical isolate CHAK36 and measure the kinetic parameters of GES-5 β-lactamase. Long-range polymerase chain reactions (PCRs) and sequence analysis were performed to identify and analyze the blaGES-5 gene cassette-containing integrons. Kinetic parameters were determined from purified GES-5. Sequencing of the 6190-bp PCR amplicon from K. pneumoniae CHAK36 isolate revealed the new structure of class 1 integron. The integron has 3 unique gene cassettes (blaGES-5-aac(6′)-IIa-blaOXA-17/orf4), but the 59-base element of the blaOXA-17 gene cassette was interrupted by a putative transposase gene, orf4. The kinetic parameters of GES-5 showed its broad-spectrum activity against most β-lactams, including benzylpenicillin, cefaloridine, cefotaxime, and imipenem. This work shows that the blaGES-5 gene was located on a new class 1 integron as a gene cassette. Our kinetic characterizations show that GES-5 was more active against impenem than GES-2 and GES-4.

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Genetic and biochemical characterization of GES-5, an extended-spectrum class A β-lactamase from Klebsiella pneumoniae

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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