Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

Dasom Mun; Ji Young Kang; Yumin Chun; Da Seul Park; Hyoeun Kim; Nuri Yun; Seung Hyun Lee; Boyoung Joung

doi:10.1016/j.scr.2022.102940

Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

Dasom Mun, Ji Young Kang, Yumin Chun, Da Seul Park, Hyoeun Kim, Nuri Yun, Seung Hyun Lee, Boyoung Joung

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

PITX2 is a homeobox gene located in the human 4q25 locus and is commonly associated with atrial fibrillation (AF). Here, we generated two PITX2 knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 genome editing. The edited iPSCs maintained full pluripotency, normal karyotype and spontaneous differentiation capability. This cell line provides a suitable model for investigating the physiopathology of PITX2 mutation in atrial fibrillation.

Original language	English
Article number	102940
Journal	Stem Cell Research
Volume	65
DOIs	https://doi.org/10.1016/j.scr.2022.102940
Publication status	Published - 2022 Dec

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea government (MSIT) (NRF-2021R1I1A1A01052197, NRF-2021R1C1C2094541); the Korean Fund for Regenerative Medicine (KFRM) grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Health & Welfare) (KFRM21B0604L1-01); and the Korean Cardiac Research Foundation (202101-02).

Publisher Copyright:
© 2022 The Authors

All Science Journal Classification (ASJC) codes

Developmental Biology
Cell Biology

Access to Document

10.1016/j.scr.2022.102940

Cite this

@article{3e2b7f2ec8454bdfbce3b3ba910fd019,

title = "Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system",

abstract = "PITX2 is a homeobox gene located in the human 4q25 locus and is commonly associated with atrial fibrillation (AF). Here, we generated two PITX2 knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 genome editing. The edited iPSCs maintained full pluripotency, normal karyotype and spontaneous differentiation capability. This cell line provides a suitable model for investigating the physiopathology of PITX2 mutation in atrial fibrillation.",

author = "Dasom Mun and Kang, {Ji Young} and Yumin Chun and Park, {Da Seul} and Hyoeun Kim and Nuri Yun and Lee, {Seung Hyun} and Boyoung Joung",

note = "Funding Information: This study was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea government (MSIT) (NRF-2021R1I1A1A01052197, NRF-2021R1C1C2094541); the Korean Fund for Regenerative Medicine (KFRM) grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Health & Welfare) (KFRM21B0604L1-01); and the Korean Cardiac Research Foundation (202101-02). Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

month = dec,

doi = "10.1016/j.scr.2022.102940",

language = "English",

volume = "65",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

}

TY - JOUR

T1 - Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

AU - Mun, Dasom

AU - Kang, Ji Young

AU - Chun, Yumin

AU - Park, Da Seul

AU - Kim, Hyoeun

AU - Yun, Nuri

AU - Lee, Seung Hyun

AU - Joung, Boyoung

N1 - Funding Information: This study was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea government (MSIT) (NRF-2021R1I1A1A01052197, NRF-2021R1C1C2094541); the Korean Fund for Regenerative Medicine (KFRM) grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Health & Welfare) (KFRM21B0604L1-01); and the Korean Cardiac Research Foundation (202101-02). Publisher Copyright: © 2022 The Authors

PY - 2022/12

Y1 - 2022/12

N2 - PITX2 is a homeobox gene located in the human 4q25 locus and is commonly associated with atrial fibrillation (AF). Here, we generated two PITX2 knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 genome editing. The edited iPSCs maintained full pluripotency, normal karyotype and spontaneous differentiation capability. This cell line provides a suitable model for investigating the physiopathology of PITX2 mutation in atrial fibrillation.

AB - PITX2 is a homeobox gene located in the human 4q25 locus and is commonly associated with atrial fibrillation (AF). Here, we generated two PITX2 knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 genome editing. The edited iPSCs maintained full pluripotency, normal karyotype and spontaneous differentiation capability. This cell line provides a suitable model for investigating the physiopathology of PITX2 mutation in atrial fibrillation.

UR - http://www.scopus.com/inward/record.url?scp=85139866004&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85139866004&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2022.102940

DO - 10.1016/j.scr.2022.102940

M3 - Article

C2 - 36260954

AN - SCOPUS:85139866004

SN - 1873-5061

VL - 65

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 102940

ER -

Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this