Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

Ji Young Kang; Dasom Mun; Yumin Chun; Da Seul Park; Hyoeun Kim; Nuri Yun; Seung Hyun Lee; Boyoung Joung

doi:10.1016/j.scr.2022.102901

Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

Ji Young Kang, Dasom Mun, Yumin Chun, Da Seul Park, Hyoeun Kim, Nuri Yun, Seung Hyun Lee, Boyoung Joung

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

TTN mutations are the common genetic cause for various types of cardiomyopathies (e.g., dilated cardiomyopathy, hypertrophic cardiomyopathy, restrictive cardiomyopathy, and arrhythmogenic right ventricular cardiomyopathy) and skeletal myopathies. Here, we generated three TTN knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 system. These cell lines, which exhibit normal karyotype, typical morphology and pluripotency, could provide useful platform for investigating the role of TTN in associated disorders.

Original language	English
Article number	102901
Journal	Stem Cell Research
Volume	64
DOIs	https://doi.org/10.1016/j.scr.2022.102901
Publication status	Published - 2022 Oct

Bibliographical note

Funding Information:
This study was supported by the Korean Fund for Regenerative Medicine (KFRM) grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Health & Welfare) (KFRM21B0604L1-01); the National Research Foundation of Korea (NRF) grants funded by the Korea government (MSIT) (NRF-2021R1I1A1A01052197 and NRF- 2021R1C1C2094541); and the Korean Cardiac Research Foundation (202101-02).

Publisher Copyright:
© 2022 The Author(s)

All Science Journal Classification (ASJC) codes

Developmental Biology
Cell Biology

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10.1016/j.scr.2022.102901

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title = "Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system",

abstract = "TTN mutations are the common genetic cause for various types of cardiomyopathies (e.g., dilated cardiomyopathy, hypertrophic cardiomyopathy, restrictive cardiomyopathy, and arrhythmogenic right ventricular cardiomyopathy) and skeletal myopathies. Here, we generated three TTN knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 system. These cell lines, which exhibit normal karyotype, typical morphology and pluripotency, could provide useful platform for investigating the role of TTN in associated disorders.",

author = "Kang, {Ji Young} and Dasom Mun and Yumin Chun and Park, {Da Seul} and Hyoeun Kim and Nuri Yun and Lee, {Seung Hyun} and Boyoung Joung",

note = "Funding Information: This study was supported by the Korean Fund for Regenerative Medicine (KFRM) grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Health & Welfare) (KFRM21B0604L1-01); the National Research Foundation of Korea (NRF) grants funded by the Korea government (MSIT) (NRF-2021R1I1A1A01052197 and NRF- 2021R1C1C2094541); and the Korean Cardiac Research Foundation (202101-02). Publisher Copyright: {\textcopyright} 2022 The Author(s)",

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doi = "10.1016/j.scr.2022.102901",

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AU - Mun, Dasom

AU - Chun, Yumin

AU - Park, Da Seul

AU - Kim, Hyoeun

AU - Yun, Nuri

AU - Lee, Seung Hyun

AU - Joung, Boyoung

N1 - Funding Information: This study was supported by the Korean Fund for Regenerative Medicine (KFRM) grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Health & Welfare) (KFRM21B0604L1-01); the National Research Foundation of Korea (NRF) grants funded by the Korea government (MSIT) (NRF-2021R1I1A1A01052197 and NRF- 2021R1C1C2094541); and the Korean Cardiac Research Foundation (202101-02). Publisher Copyright: © 2022 The Author(s)

PY - 2022/10

Y1 - 2022/10

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AB - TTN mutations are the common genetic cause for various types of cardiomyopathies (e.g., dilated cardiomyopathy, hypertrophic cardiomyopathy, restrictive cardiomyopathy, and arrhythmogenic right ventricular cardiomyopathy) and skeletal myopathies. Here, we generated three TTN knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 system. These cell lines, which exhibit normal karyotype, typical morphology and pluripotency, could provide useful platform for investigating the role of TTN in associated disorders.

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Generation of three TTN knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

Abstract

Bibliographical note

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