GC-MS Analysis of Various Phytoestrogens in Health Functional Foods

Yu Ra Kim, Hee Soo Pyo, Bong Chul Chung, Myeong Hee Moon, Jeongae Lee

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2 Citations (Scopus)


This study aimed to measure phytoestrogen contents in health functional foods (HFFs) to alleviate menopausal symptoms that are commercially available in South Korea. A sensitive and selective method using gas chromatography–mass spectrometry (GC-MS) for the monitoring of 21 phytoestrogens was developed and fully validated. Phytoestrogens were extracted by liquid–liquid extraction using methyl tert-butylether as the solvent. Food samples were enzymatically hydrolyzed with β-glucuronidase/arylsulfatase. Before GC-MS, the analytes were derivatized to their trimethylsilyl derivatives by incubation with N,O-bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane for 30 min at 80°C. The calibration curves showed good linearity (r 2 > 0.99) in the range of interest for each analyte. The limits of detection and quantification of the 21 phytoestrogens were in the ranges of 0.5–20 and 1–50 ng/mL, respectively. The intra- and inter-day precisions were all below 15.7%. These multivariate procedures were efficient in determining the optimal conditions using peak heights as responses.

Original languageEnglish
Pages (from-to)448-458
Number of pages11
JournalBulletin of the Korean Chemical Society
Issue number4
Publication statusPublished - 2017 Apr 1

Bibliographical note

Funding Information:
This research was supported by the Bio & Medical Technology Development Program through the National Research Foundation of Korea funded by the Ministry of Science, ICT and Future Planning (NRF-2013M3A9B6046413) and by an intramural grant from the Korea Institute of Science and Technology (2E25360)

Publisher Copyright:
© 2017 Korean Chemical Society, Seoul & Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

All Science Journal Classification (ASJC) codes

  • Chemistry(all)


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