Gap junctions contribute to astrocytic resistance against zinc toxicity

Jinu Lee, Yeong Shin Yim, Suk Jin Ko, Dong Goo Kim, Chul Hoon Kim

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8 Citations (Scopus)


Astrocytic gap junctions have been implicated in the regulation of cell viability. High amounts of extracellular zinc, which is released during ischemia, seizure, and brain trauma, can be cytotoxic to astrocytes. We tested whether gap junction coupling between astrocytes plays an important role in modulating zinc toxicity in hippocampal astrocytes. Zinc induces cell death in a dose-dependent manner in primary cultured hippocampal astrocytes. Two gap junction inhibitors, 18β-glycyrrhetinic acid and arachidonic acid, had no effect on zinc-induced cell death in low-confluence culture, where physical separation prevents gap junctions from forming. However, these inhibitors can potentiate zinc toxicity in high-confluence astrocyte cultures. Zinc toxicity was substantially suppressed upon connexin 43 overexpression, whereas knockdown caused a significant enhancement of the toxicity in high-confluence cultures. These data suggest that gap junctions in hippocampal astrocytes provide a protective role against zinc toxicity.

Original languageEnglish
Pages (from-to)314-318
Number of pages5
JournalBrain Research Bulletin
Issue number5-6
Publication statusPublished - 2011 Nov 25

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government ( 331-2008-1-E00056 ) to C.H. Kim. We thank the Yonsei-Carl Zeiss Advanced Imaging Center, Yonsei University College of Medicine, for technical assistance.

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)


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