Functional restoration of pituitary after pituitary allotransplantation into hypophysectomized rats

Jai Ho Choi, Jung Eun Lee, Hong Lim Kim, Seung Hyun Ko, Se Hoon Kim, Seung Ho Yang

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1 Citation (Scopus)

Abstract

Long-term hormone replacement therapy due to panhypopituitarism can lead to serious complications and thus, pituitary transplantation is considered a more desirable. We investigated functional restoration after allotransplatation of the pituitary gland. We transplanted extracted pituitary gland into the omentum of an hypophysectomized rat. Two experiments were performed: (1) to confirm the hypophysectomy was successful and (2) to assess functional restoration after pituitary transplantation. Pituitary hormone level and weight change were consecutively assessed. Electron microscopic (EM) examinations were performed to identify morphological changes at 3 days after transplantation. We confirmed that pituitary gland was properly extracted from 6 rats after sacrifice. The findings showed (1) a weight loss of more than 3% or (2) a weight change of less than 2% along with a decreased growth hormone (GH) level by more than 80% at 2 weeks posthypophysectomy. A further four rats underwent pituitary transplantation after hypophysectomy and were compared with the previously hypophysectomized rats. All showed rapid weight gain during the two weeks after transplantation. The thyroid-stimulating hormone, prolactin, and GH levels were restored at one week post-transplantation and maintained for 10 weeks. Hypophyseal tissue architecture was maintained at 3 days after transplantation, as indicated by EM. These data suggest that a transplanted pituitary gland can survive in the omentum with concomitant partial restoration of anterior pituitary hormones.

Original languageEnglish
Article number267
Pages (from-to)1-11
Number of pages11
JournalCells
Volume10
Issue number2
DOIs
Publication statusPublished - 2021 Feb

Bibliographical note

Publisher Copyright:
© 2021 by the authors.

All Science Journal Classification (ASJC) codes

  • General Biochemistry,Genetics and Molecular Biology

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