Functional Identification of OphR, an IclR Family Transcriptional Regulator Involved in the Regulation of the Phthalate Catabolic Operon in Rhodococcus sp. Strain DK17

Ki Young Choi; Beom Sik Kang; Myung Hee Nam; Woo Jun Sul; Eungbin Kim

doi:10.1007/s12088-015-0529-5

Functional Identification of OphR, an IclR Family Transcriptional Regulator Involved in the Regulation of the Phthalate Catabolic Operon in Rhodococcus sp. Strain DK17

Ki Young Choi, Beom Sik Kang, Myung Hee Nam, Woo Jun Sul, Eungbin Kim

Department of Systems Biology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A putative gene for a transcriptional regulator (ophR) was detected near each copy of the duplicated phthalate-degrading operon of Rhodococcus sp. DK17. Sequence analysis and molecular modeling indicate that OphR belongs to the IclR family of transcriptional regulators and possesses the N-terminal DNA-binding and C-terminal effector-binding domains. DNA-binding assays demonstrate that OphR regulates the phthalate operon by binding to the ophA1-ophR intergenic region.

Original language	English
Pages (from-to)	313-318
Number of pages	6
Journal	Indian Journal of Microbiology
Volume	55
Issue number	3
DOIs	https://doi.org/10.1007/s12088-015-0529-5
Publication status	Published - 2015 Sept 6

Bibliographical note

Funding Information:
This work was financially supported by a grant from the Ministry of Oceans and Fisheries, Korea to the Korea Polar Research Institute (Project PM13030).

Publisher Copyright:
© 2015, Association of Microbiologists of India.

All Science Journal Classification (ASJC) codes

Microbiology

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title = "Functional Identification of OphR, an IclR Family Transcriptional Regulator Involved in the Regulation of the Phthalate Catabolic Operon in Rhodococcus sp. Strain DK17",

abstract = "A putative gene for a transcriptional regulator (ophR) was detected near each copy of the duplicated phthalate-degrading operon of Rhodococcus sp. DK17. Sequence analysis and molecular modeling indicate that OphR belongs to the IclR family of transcriptional regulators and possesses the N-terminal DNA-binding and C-terminal effector-binding domains. DNA-binding assays demonstrate that OphR regulates the phthalate operon by binding to the ophA1-ophR intergenic region.",

author = "Choi, {Ki Young} and Kang, {Beom Sik} and Nam, {Myung Hee} and Sul, {Woo Jun} and Eungbin Kim",

note = "Funding Information: This work was financially supported by a grant from the Ministry of Oceans and Fisheries, Korea to the Korea Polar Research Institute (Project PM13030). Publisher Copyright: {\textcopyright} 2015, Association of Microbiologists of India.",

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doi = "10.1007/s12088-015-0529-5",

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T1 - Functional Identification of OphR, an IclR Family Transcriptional Regulator Involved in the Regulation of the Phthalate Catabolic Operon in Rhodococcus sp. Strain DK17

AU - Choi, Ki Young

AU - Kang, Beom Sik

AU - Nam, Myung Hee

AU - Sul, Woo Jun

AU - Kim, Eungbin

N1 - Funding Information: This work was financially supported by a grant from the Ministry of Oceans and Fisheries, Korea to the Korea Polar Research Institute (Project PM13030). Publisher Copyright: © 2015, Association of Microbiologists of India.

PY - 2015/9/6

Y1 - 2015/9/6

N2 - A putative gene for a transcriptional regulator (ophR) was detected near each copy of the duplicated phthalate-degrading operon of Rhodococcus sp. DK17. Sequence analysis and molecular modeling indicate that OphR belongs to the IclR family of transcriptional regulators and possesses the N-terminal DNA-binding and C-terminal effector-binding domains. DNA-binding assays demonstrate that OphR regulates the phthalate operon by binding to the ophA1-ophR intergenic region.

AB - A putative gene for a transcriptional regulator (ophR) was detected near each copy of the duplicated phthalate-degrading operon of Rhodococcus sp. DK17. Sequence analysis and molecular modeling indicate that OphR belongs to the IclR family of transcriptional regulators and possesses the N-terminal DNA-binding and C-terminal effector-binding domains. DNA-binding assays demonstrate that OphR regulates the phthalate operon by binding to the ophA1-ophR intergenic region.

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Functional Identification of OphR, an IclR Family Transcriptional Regulator Involved in the Regulation of the Phthalate Catabolic Operon in Rhodococcus sp. Strain DK17

Abstract

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