Full -wave modelling of two-photon microscopy with spatiotemporal focussing

Philip Wijesinghe; Kishan Dholakia; R. T. Peter Munro

doi:10.1109/IPC53466.2022.9975671

Full -wave modelling of two-photon microscopy with spatiotemporal focussing

Philip Wijesinghe, Kishan Dholakia, R. T. Peter Munro

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Spatiotemporal focussing has enabled widefield, axially confined multiphoton excitation. Its main advantage of an improved performance through scattering media, useful for deep imaging and optogenetics, however, has largely been evaluated empirically because of a lack of a suitable computational model. To overcome this we have developed a full-wave simulation describing two-photon microscopy image formation through scattering media with spatiotemporal focussing. This model provides full freedom to vary imaging parameters and observe quantities not generally accessible in experiment. We use this model to reveal new insights into the properties of spatiotemporal focussing in the presence of scattering media.

Original language	English
Title of host publication	2022 IEEE Photonics Conference, IPC 2022 - Proceedings
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781665434874
DOIs	https://doi.org/10.1109/IPC53466.2022.9975671
Publication status	Published - 2022
Event	2022 IEEE Photonics Conference, IPC 2022 - Vancouver, Canada Duration: 2022 Nov 13 → 2022 Nov 17

Publication series

Name	2022 IEEE Photonics Conference, IPC 2022 - Proceedings

Conference

Conference	2022 IEEE Photonics Conference, IPC 2022
Country/Territory	Canada
City	Vancouver
Period	22/11/13 → 22/11/17

Bibliographical note

Publisher Copyright:
© 2022 The Author(s)

All Science Journal Classification (ASJC) codes

Control and Optimization
Instrumentation
Atomic and Molecular Physics, and Optics
Artificial Intelligence
Computer Networks and Communications
Electrical and Electronic Engineering
Global and Planetary Change
Management, Monitoring, Policy and Law

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10.1109/IPC53466.2022.9975671

Cite this

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title = "Full -wave modelling of two-photon microscopy with spatiotemporal focussing",

abstract = "Spatiotemporal focussing has enabled widefield, axially confined multiphoton excitation. Its main advantage of an improved performance through scattering media, useful for deep imaging and optogenetics, however, has largely been evaluated empirically because of a lack of a suitable computational model. To overcome this we have developed a full-wave simulation describing two-photon microscopy image formation through scattering media with spatiotemporal focussing. This model provides full freedom to vary imaging parameters and observe quantities not generally accessible in experiment. We use this model to reveal new insights into the properties of spatiotemporal focussing in the presence of scattering media.",

author = "Philip Wijesinghe and Kishan Dholakia and {Peter Munro}, {R. T.}",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s); 2022 IEEE Photonics Conference, IPC 2022 ; Conference date: 13-11-2022 Through 17-11-2022",

year = "2022",

doi = "10.1109/IPC53466.2022.9975671",

language = "English",

series = "2022 IEEE Photonics Conference, IPC 2022 - Proceedings",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

booktitle = "2022 IEEE Photonics Conference, IPC 2022 - Proceedings",

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Wijesinghe, P, Dholakia, K & Peter Munro, RT 2022, Full -wave modelling of two-photon microscopy with spatiotemporal focussing. in 2022 IEEE Photonics Conference, IPC 2022 - Proceedings. 2022 IEEE Photonics Conference, IPC 2022 - Proceedings, Institute of Electrical and Electronics Engineers Inc., 2022 IEEE Photonics Conference, IPC 2022, Vancouver, Canada, 22/11/13. https://doi.org/10.1109/IPC53466.2022.9975671

Full -wave modelling of two-photon microscopy with spatiotemporal focussing. / Wijesinghe, Philip; Dholakia, Kishan; Peter Munro, R. T.
2022 IEEE Photonics Conference, IPC 2022 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2022. (2022 IEEE Photonics Conference, IPC 2022 - Proceedings).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Full -wave modelling of two-photon microscopy with spatiotemporal focussing

AU - Wijesinghe, Philip

AU - Dholakia, Kishan

AU - Peter Munro, R. T.

PY - 2022

Y1 - 2022

N2 - Spatiotemporal focussing has enabled widefield, axially confined multiphoton excitation. Its main advantage of an improved performance through scattering media, useful for deep imaging and optogenetics, however, has largely been evaluated empirically because of a lack of a suitable computational model. To overcome this we have developed a full-wave simulation describing two-photon microscopy image formation through scattering media with spatiotemporal focussing. This model provides full freedom to vary imaging parameters and observe quantities not generally accessible in experiment. We use this model to reveal new insights into the properties of spatiotemporal focussing in the presence of scattering media.

AB - Spatiotemporal focussing has enabled widefield, axially confined multiphoton excitation. Its main advantage of an improved performance through scattering media, useful for deep imaging and optogenetics, however, has largely been evaluated empirically because of a lack of a suitable computational model. To overcome this we have developed a full-wave simulation describing two-photon microscopy image formation through scattering media with spatiotemporal focussing. This model provides full freedom to vary imaging parameters and observe quantities not generally accessible in experiment. We use this model to reveal new insights into the properties of spatiotemporal focussing in the presence of scattering media.

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DO - 10.1109/IPC53466.2022.9975671

M3 - Conference contribution

AN - SCOPUS:85145617452

T3 - 2022 IEEE Photonics Conference, IPC 2022 - Proceedings

BT - 2022 IEEE Photonics Conference, IPC 2022 - Proceedings

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 2022 IEEE Photonics Conference, IPC 2022

Y2 - 13 November 2022 through 17 November 2022

ER -

Full -wave modelling of two-photon microscopy with spatiotemporal focussing

Abstract

Publication series

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Bibliographical note

All Science Journal Classification (ASJC) codes

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