Full -wave modelling of two-photon microscopy with spatiotemporal focussing

Philip Wijesinghe, Kishan Dholakia, R. T. Peter Munro

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Spatiotemporal focussing has enabled widefield, axially confined multiphoton excitation. Its main advantage of an improved performance through scattering media, useful for deep imaging and optogenetics, however, has largely been evaluated empirically because of a lack of a suitable computational model. To overcome this we have developed a full-wave simulation describing two-photon microscopy image formation through scattering media with spatiotemporal focussing. This model provides full freedom to vary imaging parameters and observe quantities not generally accessible in experiment. We use this model to reveal new insights into the properties of spatiotemporal focussing in the presence of scattering media.

Original languageEnglish
Title of host publication2022 IEEE Photonics Conference, IPC 2022 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781665434874
Publication statusPublished - 2022
Event2022 IEEE Photonics Conference, IPC 2022 - Vancouver, Canada
Duration: 2022 Nov 132022 Nov 17

Publication series

Name2022 IEEE Photonics Conference, IPC 2022 - Proceedings


Conference2022 IEEE Photonics Conference, IPC 2022

Bibliographical note

Publisher Copyright:
© 2022 The Author(s)

All Science Journal Classification (ASJC) codes

  • Control and Optimization
  • Instrumentation
  • Atomic and Molecular Physics, and Optics
  • Artificial Intelligence
  • Computer Networks and Communications
  • Electrical and Electronic Engineering
  • Global and Planetary Change
  • Management, Monitoring, Policy and Law


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