Follistatin mitigates myofibroblast differentiation and collagen synthesis of fibroblasts from scar tissue around injured flexor tendons

Young Mi Kang, Su Keon Lee, Yong Min Chun, Yun Rak Choi, Seong Hwan Moon, Hwan Mo Lee, Ho Jung Kang

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Purpose: The aim of this study was to investigate the effect of FST gene on the inhibition of fibrosis in fibroblastic cells from scar tissue around repaired zone II flexor tendons. Materials and Methods: Immunohistochemistry was conducted on fibroblast cells transfected with adenovirus-LacZ (Ad-LacZ) as a marker gene (control), or with adenovirus-FST (Ad-FST) as a therapeutic gene. Fibroblast cultures without adenoviral exposure served as controls. Results: Fibroblastic cells transfected with Ad-FST demonstrated significant decrease in collagen type I, MMP-1, MMP2, and α-SMA mRNA expressions compared to those transfected with Ad-LacZ. In addition, fibroblastic cells transfected with Ad-FST exhibited significant decrease in MMP-1, TIMP-1, fibronectin, PAI-1, TRPV4, α-SMA, desmin, and PAX7 protein expressions. Conclusion: Based on these findings, we conclude that FST may be a novel therapeutic strategy for preventing scar adhesions around repaired tendons by inhibiting fibroblasts from differentiating into myofibroblasts, in addition to producing type I collagen and regulating extracellular matrix turnover via the downregulation of MMP-1 and TIMP-1. FST may also decrease contracture of the scar by inhibiting Ca2+-dependent cell contraction.

Original languageEnglish
Pages (from-to)85-93
Number of pages9
JournalYonsei medical journal
Volume61
Issue number1
DOIs
Publication statusPublished - 2020

Bibliographical note

Publisher Copyright:
© Yonsei University College of Medicine 2020 This is an Open Access article distributed under the terms of the.

All Science Journal Classification (ASJC) codes

  • General Medicine

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