Fluorescence lifetime microscopy for monitoring cell adhesion using metal induced energy transfer

Wonsang Hwang; Jinwon Seo; Jun Ho Song; Dongeun Kim; Youngjae Won; In Hong Choi; Kyung Hwa Yoo; Dug Young Kim

doi:10.1117/12.2289330

Fluorescence lifetime microscopy for monitoring cell adhesion using metal induced energy transfer

Wonsang Hwang, Jinwon Seo, Jun Ho Song, Dongeun Kim, Youngjae Won, In Hong Choi, Kyung Hwa Yoo, Dug Young Kim

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A precise control and a reliable monitoring tool for the adhesion properties of a cell are very important in atherosclerosis studies. If endothelial cells in contact with the intracellular membrane are not attached securely, low-density lipoprotein (LDL) particles can enter into the inner membrane. It is therefore necessary to measure conditions under which endothelial cell detachment occurs. When a cell is attached to a metal thin film, the lifetime of a fluorescence probe attached to the membrane of the cell is reduced by the metal-induced energy transfer (MIET). Fluorescence lifetime imaging microscopy (FLIM) is used to monitor the attachment condition of a cell to a metal surface using FRET. However, this requires high numerical aperture (NA) objective lens because axial confocal resolution must be smaller than the cell thickness. This requirement limits the field of view of the measurement specimen. In this study we provides a new method which can measure adhesion properties of endothelial cells even with a low NA objective lens by resolving two lifetime components in FLIM.

Original language	English
Title of host publication	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI
Editors	Dan V. Nicolau, Daniel L. Farkas, Robert C. Leif
Publisher	SPIE
ISBN (Electronic)	9781510614796
DOIs	https://doi.org/10.1117/12.2289330
Publication status	Published - 2018
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI 2018 - San Francisco, United States Duration: 2018 Jan 29 → 2018 Jan 31

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10497
ISSN (Print)	1605-7422

Other

Other	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI 2018
Country/Territory	United States
City	San Francisco
Period	18/1/29 → 18/1/31

Bibliographical note

Funding Information:
This work was financially supported by the National Research Foundation of Korea (NRF-2013R1A1A2062448) through the Basic Science Research Program, Global Frontier Project (CAMM-2014M3A6B3063712), the Ministry of Science and ICT of Korea, Technology Innovation Program (10062417), and the Ministry of Trade, Industry and Energy of Korea.

Publisher Copyright:
© 2018 SPIE.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2289330

Cite this

Hwang, W., Seo, J., Song, J. H., Kim, D., Won, Y., Choi, I. H., Yoo, K. H., & Kim, D. Y. (2018). Fluorescence lifetime microscopy for monitoring cell adhesion using metal induced energy transfer. In D. V. Nicolau, D. L. Farkas, & R. C. Leif (Eds.), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI Article 104970E (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10497). SPIE. https://doi.org/10.1117/12.2289330

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abstract = "A precise control and a reliable monitoring tool for the adhesion properties of a cell are very important in atherosclerosis studies. If endothelial cells in contact with the intracellular membrane are not attached securely, low-density lipoprotein (LDL) particles can enter into the inner membrane. It is therefore necessary to measure conditions under which endothelial cell detachment occurs. When a cell is attached to a metal thin film, the lifetime of a fluorescence probe attached to the membrane of the cell is reduced by the metal-induced energy transfer (MIET). Fluorescence lifetime imaging microscopy (FLIM) is used to monitor the attachment condition of a cell to a metal surface using FRET. However, this requires high numerical aperture (NA) objective lens because axial confocal resolution must be smaller than the cell thickness. This requirement limits the field of view of the measurement specimen. In this study we provides a new method which can measure adhesion properties of endothelial cells even with a low NA objective lens by resolving two lifetime components in FLIM.",

author = "Wonsang Hwang and Jinwon Seo and Song, {Jun Ho} and Dongeun Kim and Youngjae Won and Choi, {In Hong} and Yoo, {Kyung Hwa} and Kim, {Dug Young}",

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Hwang, W, Seo, J, Song, JH, Kim, D, Won, Y, Choi, IH, Yoo, KH & Kim, DY 2018, Fluorescence lifetime microscopy for monitoring cell adhesion using metal induced energy transfer. in DV Nicolau, DL Farkas & RC Leif (eds), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI., 104970E, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10497, SPIE, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI 2018, San Francisco, United States, 18/1/29. https://doi.org/10.1117/12.2289330

Fluorescence lifetime microscopy for monitoring cell adhesion using metal induced energy transfer. / Hwang, Wonsang; Seo, Jinwon; Song, Jun Ho et al.
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI. ed. / Dan V. Nicolau; Daniel L. Farkas; Robert C. Leif. SPIE, 2018. 104970E (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10497).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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T1 - Fluorescence lifetime microscopy for monitoring cell adhesion using metal induced energy transfer

AU - Hwang, Wonsang

AU - Seo, Jinwon

AU - Song, Jun Ho

AU - Kim, Dongeun

AU - Won, Youngjae

AU - Choi, In Hong

AU - Yoo, Kyung Hwa

AU - Kim, Dug Young

N1 - Funding Information: This work was financially supported by the National Research Foundation of Korea (NRF-2013R1A1A2062448) through the Basic Science Research Program, Global Frontier Project (CAMM-2014M3A6B3063712), the Ministry of Science and ICT of Korea, Technology Innovation Program (10062417), and the Ministry of Trade, Industry and Energy of Korea. Publisher Copyright: © 2018 SPIE.

PY - 2018

Y1 - 2018

N2 - A precise control and a reliable monitoring tool for the adhesion properties of a cell are very important in atherosclerosis studies. If endothelial cells in contact with the intracellular membrane are not attached securely, low-density lipoprotein (LDL) particles can enter into the inner membrane. It is therefore necessary to measure conditions under which endothelial cell detachment occurs. When a cell is attached to a metal thin film, the lifetime of a fluorescence probe attached to the membrane of the cell is reduced by the metal-induced energy transfer (MIET). Fluorescence lifetime imaging microscopy (FLIM) is used to monitor the attachment condition of a cell to a metal surface using FRET. However, this requires high numerical aperture (NA) objective lens because axial confocal resolution must be smaller than the cell thickness. This requirement limits the field of view of the measurement specimen. In this study we provides a new method which can measure adhesion properties of endothelial cells even with a low NA objective lens by resolving two lifetime components in FLIM.

AB - A precise control and a reliable monitoring tool for the adhesion properties of a cell are very important in atherosclerosis studies. If endothelial cells in contact with the intracellular membrane are not attached securely, low-density lipoprotein (LDL) particles can enter into the inner membrane. It is therefore necessary to measure conditions under which endothelial cell detachment occurs. When a cell is attached to a metal thin film, the lifetime of a fluorescence probe attached to the membrane of the cell is reduced by the metal-induced energy transfer (MIET). Fluorescence lifetime imaging microscopy (FLIM) is used to monitor the attachment condition of a cell to a metal surface using FRET. However, this requires high numerical aperture (NA) objective lens because axial confocal resolution must be smaller than the cell thickness. This requirement limits the field of view of the measurement specimen. In this study we provides a new method which can measure adhesion properties of endothelial cells even with a low NA objective lens by resolving two lifetime components in FLIM.

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M3 - Conference contribution

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BT - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI

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Hwang W, Seo J, Song JH, Kim D, Won Y, Choi IH et al. Fluorescence lifetime microscopy for monitoring cell adhesion using metal induced energy transfer. In Nicolau DV, Farkas DL, Leif RC, editors, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI. SPIE. 2018. 104970E. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2289330

Fluorescence lifetime microscopy for monitoring cell adhesion using metal induced energy transfer

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Bibliographical note

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