Fluorescence immunoassay of anti-cyclic citrulinated peptide (CCP) autoantibodies by using parylene-H film

Hyuk Ko; Ga yeon Lee; Byoung Jin Jeon; Jae Chul Pyun

doi:10.1007/s13206-011-5308-8

Fluorescence immunoassay of anti-cyclic citrulinated peptide (CCP) autoantibodies by using parylene-H film

Hyuk Ko, Ga yeon Lee, Byoung Jin Jeon, Jae Chul Pyun

Department of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

A fluorescence immunoassay for rheumatoid arthritis was presented by immobilizing a short peptide cyclic citrullinated peptide (CCP) through parylene-H film. The parylene-H film is a polymer of pxylene modified with formyl groups which can react with primary amine groups of proteins or peptides. In this work, the covalent coupling of the peptide can be produced by only one step of incubation without additional coupling reagent, and the immobilization efficiency to the parylene-H film was compared with the immobilization of CCP through conventional physical adsorption. The applicability of this immobilization method for short peptides is demonstrated by detecting autoantibodies in rheumatoid arthritis patient serum.

Original language	English
Pages (from-to)	242-245
Number of pages	4
Journal	Biochip Journal
Volume	5
Issue number	3
DOIs	https://doi.org/10.1007/s13206-011-5308-8
Publication status	Published - 2011 Sept

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomedical Engineering
Electrical and Electronic Engineering

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abstract = "A fluorescence immunoassay for rheumatoid arthritis was presented by immobilizing a short peptide cyclic citrullinated peptide (CCP) through parylene-H film. The parylene-H film is a polymer of pxylene modified with formyl groups which can react with primary amine groups of proteins or peptides. In this work, the covalent coupling of the peptide can be produced by only one step of incubation without additional coupling reagent, and the immobilization efficiency to the parylene-H film was compared with the immobilization of CCP through conventional physical adsorption. The applicability of this immobilization method for short peptides is demonstrated by detecting autoantibodies in rheumatoid arthritis patient serum.",

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AU - Pyun, Jae Chul

PY - 2011/9

Y1 - 2011/9

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AB - A fluorescence immunoassay for rheumatoid arthritis was presented by immobilizing a short peptide cyclic citrullinated peptide (CCP) through parylene-H film. The parylene-H film is a polymer of pxylene modified with formyl groups which can react with primary amine groups of proteins or peptides. In this work, the covalent coupling of the peptide can be produced by only one step of incubation without additional coupling reagent, and the immobilization efficiency to the parylene-H film was compared with the immobilization of CCP through conventional physical adsorption. The applicability of this immobilization method for short peptides is demonstrated by detecting autoantibodies in rheumatoid arthritis patient serum.

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Fluorescence immunoassay of anti-cyclic citrulinated peptide (CCP) autoantibodies by using parylene-H film

Abstract

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