Fluorescence-based Quantification of Bioactive Keratin Peptides from Feathers for Optimizing Large-scale Anaerobic Fermentation and Purification

Hyeon Su Jin; Seon Yeong Park; Ji Yeon Kim; Jae Eun Lee; Han Seung Lee; Nam Joo Kang; Dong Woo Lee

doi:10.1007/s12257-018-0400-8

Fluorescence-based Quantification of Bioactive Keratin Peptides from Feathers for Optimizing Large-scale Anaerobic Fermentation and Purification

Hyeon Su Jin, Seon Yeong Park, Ji Yeon Kim, Jae Eun Lee, Han Seung Lee, Nam Joo Kang, Dong Woo Lee

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The extremely thermophilic eubacterium Fervidobacterium islandicum AW-1 produces low molecular weight (LMW; < 1 kDa) keratin peptides (KPs) from poultry feathers at 70°C. However, detection and quantification of feather hydrolysate-derived peptides is needed for optimizing fermentation and down-stream processes. Herein, we developed a large-scale fermentation and purification of skin anti-aging LMW KPs from recalcitrant feathers using fluorescence-based quantification of N-terminal prolinecontaining KPs derivatized with 3,4-dihydroxybenzoic acid to yield fluorescent adducts. Fluorescent products were correlated with bioactive KP concentrations in keratin fractions and cosmetic formulations. Subsequent anaerobic fermentative keratinolysis and large-scale purification achieved 4.4 g/L LMW KPs from 8 g/L native feathers in a 5 L batch bioreactor, generating 0.8 g/L purified MMP-1 suppressive KPs (yield = 1.2%). This demonstrated the feasibility of industrial-scale anaerobic feather digestion and purification of LMW KPs to produce skin anti-aging peptides from keratin hydrolysates in a more environmentally sustainable manner.

Original language	English
Pages (from-to)	240-249
Number of pages	10
Journal	Biotechnology and Bioprocess Engineering
Volume	24
Issue number	1
DOIs	https://doi.org/10.1007/s12257-018-0400-8
Publication status	Published - 2019 Feb 1

Bibliographical note

Funding Information:
This work was supported by the National Coordinating Center for Global Cosmetics R&D (NCR) via grant no. HN14C0097 to NJ Kang, funded by the Ministry of Health and Welfare; by the National Research Foundation of Korea (NRF) via grant no. 2017R1A2B4005051 to DW Lee, funded by the Ministry of Science, ICT and Future Planning; the Strategic Initiative for Microbiomes in Agriculture and Food via grant no. 918012-4 to DW Lee, funded by the Ministry of Agriculture, Food, and Rural Affairs; and by the NRF via grant no. 2016R1D1A1B04934489 to HS Lee, funded by the Ministry of Education. The authors declare no conflict of interest.

Publisher Copyright:
© 2019, The Korean Society for Biotechnology and Bioengineering and Springer.

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

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10.1007/s12257-018-0400-8

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title = "Fluorescence-based Quantification of Bioactive Keratin Peptides from Feathers for Optimizing Large-scale Anaerobic Fermentation and Purification",

abstract = "The extremely thermophilic eubacterium Fervidobacterium islandicum AW-1 produces low molecular weight (LMW; < 1 kDa) keratin peptides (KPs) from poultry feathers at 70°C. However, detection and quantification of feather hydrolysate-derived peptides is needed for optimizing fermentation and down-stream processes. Herein, we developed a large-scale fermentation and purification of skin anti-aging LMW KPs from recalcitrant feathers using fluorescence-based quantification of N-terminal prolinecontaining KPs derivatized with 3,4-dihydroxybenzoic acid to yield fluorescent adducts. Fluorescent products were correlated with bioactive KP concentrations in keratin fractions and cosmetic formulations. Subsequent anaerobic fermentative keratinolysis and large-scale purification achieved 4.4 g/L LMW KPs from 8 g/L native feathers in a 5 L batch bioreactor, generating 0.8 g/L purified MMP-1 suppressive KPs (yield = 1.2%). This demonstrated the feasibility of industrial-scale anaerobic feather digestion and purification of LMW KPs to produce skin anti-aging peptides from keratin hydrolysates in a more environmentally sustainable manner.",

author = "Jin, {Hyeon Su} and Park, {Seon Yeong} and Kim, {Ji Yeon} and Lee, {Jae Eun} and Lee, {Han Seung} and Kang, {Nam Joo} and Lee, {Dong Woo}",

note = "Funding Information: This work was supported by the National Coordinating Center for Global Cosmetics R&D (NCR) via grant no. HN14C0097 to NJ Kang, funded by the Ministry of Health and Welfare; by the National Research Foundation of Korea (NRF) via grant no. 2017R1A2B4005051 to DW Lee, funded by the Ministry of Science, ICT and Future Planning; the Strategic Initiative for Microbiomes in Agriculture and Food via grant no. 918012-4 to DW Lee, funded by the Ministry of Agriculture, Food, and Rural Affairs; and by the NRF via grant no. 2016R1D1A1B04934489 to HS Lee, funded by the Ministry of Education. The authors declare no conflict of interest. Publisher Copyright: {\textcopyright} 2019, The Korean Society for Biotechnology and Bioengineering and Springer.",

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AU - Jin, Hyeon Su

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AU - Lee, Jae Eun

AU - Lee, Han Seung

AU - Kang, Nam Joo

AU - Lee, Dong Woo

N1 - Funding Information: This work was supported by the National Coordinating Center for Global Cosmetics R&D (NCR) via grant no. HN14C0097 to NJ Kang, funded by the Ministry of Health and Welfare; by the National Research Foundation of Korea (NRF) via grant no. 2017R1A2B4005051 to DW Lee, funded by the Ministry of Science, ICT and Future Planning; the Strategic Initiative for Microbiomes in Agriculture and Food via grant no. 918012-4 to DW Lee, funded by the Ministry of Agriculture, Food, and Rural Affairs; and by the NRF via grant no. 2016R1D1A1B04934489 to HS Lee, funded by the Ministry of Education. The authors declare no conflict of interest. Publisher Copyright: © 2019, The Korean Society for Biotechnology and Bioengineering and Springer.

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N2 - The extremely thermophilic eubacterium Fervidobacterium islandicum AW-1 produces low molecular weight (LMW; < 1 kDa) keratin peptides (KPs) from poultry feathers at 70°C. However, detection and quantification of feather hydrolysate-derived peptides is needed for optimizing fermentation and down-stream processes. Herein, we developed a large-scale fermentation and purification of skin anti-aging LMW KPs from recalcitrant feathers using fluorescence-based quantification of N-terminal prolinecontaining KPs derivatized with 3,4-dihydroxybenzoic acid to yield fluorescent adducts. Fluorescent products were correlated with bioactive KP concentrations in keratin fractions and cosmetic formulations. Subsequent anaerobic fermentative keratinolysis and large-scale purification achieved 4.4 g/L LMW KPs from 8 g/L native feathers in a 5 L batch bioreactor, generating 0.8 g/L purified MMP-1 suppressive KPs (yield = 1.2%). This demonstrated the feasibility of industrial-scale anaerobic feather digestion and purification of LMW KPs to produce skin anti-aging peptides from keratin hydrolysates in a more environmentally sustainable manner.

AB - The extremely thermophilic eubacterium Fervidobacterium islandicum AW-1 produces low molecular weight (LMW; < 1 kDa) keratin peptides (KPs) from poultry feathers at 70°C. However, detection and quantification of feather hydrolysate-derived peptides is needed for optimizing fermentation and down-stream processes. Herein, we developed a large-scale fermentation and purification of skin anti-aging LMW KPs from recalcitrant feathers using fluorescence-based quantification of N-terminal prolinecontaining KPs derivatized with 3,4-dihydroxybenzoic acid to yield fluorescent adducts. Fluorescent products were correlated with bioactive KP concentrations in keratin fractions and cosmetic formulations. Subsequent anaerobic fermentative keratinolysis and large-scale purification achieved 4.4 g/L LMW KPs from 8 g/L native feathers in a 5 L batch bioreactor, generating 0.8 g/L purified MMP-1 suppressive KPs (yield = 1.2%). This demonstrated the feasibility of industrial-scale anaerobic feather digestion and purification of LMW KPs to produce skin anti-aging peptides from keratin hydrolysates in a more environmentally sustainable manner.

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Fluorescence-based Quantification of Bioactive Keratin Peptides from Feathers for Optimizing Large-scale Anaerobic Fermentation and Purification

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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