Abstract
The extremely thermophilic eubacterium Fervidobacterium islandicum AW-1 produces low molecular weight (LMW; < 1 kDa) keratin peptides (KPs) from poultry feathers at 70°C. However, detection and quantification of feather hydrolysate-derived peptides is needed for optimizing fermentation and down-stream processes. Herein, we developed a large-scale fermentation and purification of skin anti-aging LMW KPs from recalcitrant feathers using fluorescence-based quantification of N-terminal prolinecontaining KPs derivatized with 3,4-dihydroxybenzoic acid to yield fluorescent adducts. Fluorescent products were correlated with bioactive KP concentrations in keratin fractions and cosmetic formulations. Subsequent anaerobic fermentative keratinolysis and large-scale purification achieved 4.4 g/L LMW KPs from 8 g/L native feathers in a 5 L batch bioreactor, generating 0.8 g/L purified MMP-1 suppressive KPs (yield = 1.2%). This demonstrated the feasibility of industrial-scale anaerobic feather digestion and purification of LMW KPs to produce skin anti-aging peptides from keratin hydrolysates in a more environmentally sustainable manner.
Original language | English |
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Pages (from-to) | 240-249 |
Number of pages | 10 |
Journal | Biotechnology and Bioprocess Engineering |
Volume | 24 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2019 Feb 1 |
Bibliographical note
Publisher Copyright:© 2019, The Korean Society for Biotechnology and Bioengineering and Springer.
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Biomedical Engineering