Fibrillarin binds to a 3′ cis-regulatory element in pre-mRNA of uvi15+ in fission yeast

Yeun Kyu Jang, Minkyu Kim, Sang Dai Park

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3 Citations (Scopus)


uvi15+ is induced by various stresses including exposure to UV-light. Previously, we demonstrated that the UV-induction is mainly regulated at the post-transcriptional level through a cis-acting element in the pre-mRNA. Here we show that deletion analyses define an 18-nt element responsible for the UV-induction. RNA gel mobility shift assay showed that a specific protein(s) could form a complex with the 54-nt element but its binding ability is moderately decreased in response to UV-light. Using yeast three-hybrid screen, we isolated a homolog of fibrillarin as a protein interacting with the 54-nt element, which is a key nucleolar protein for pre-rRNA processing. We further showed that the recombinant fibrillarin specifically binds to the element in a sequence-specific manner. Thus, the data suggest that fission yeast fibrillarin might regulate uvi15+ mRNA stability via binding with the 54-nt element in the pre-mRNA, implying that fibrillarin is involved in both pre-mRNA and pre-rRNA processing.

Original languageEnglish
Pages (from-to)1184-1190
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number5
Publication statusPublished - 2002

Bibliographical note

Funding Information:
We thank Drs. Marvin Wickens and M. Yamamoto for providing yeast strains and plasmids used in three-hybrid assay and S. pombe cell strain, respectively. We also thank Dr. O. Hwang for critical reading of the manuscript. This work was supported in part by a grant for the leading scientists from the Korea Science and Engineering Foundation (2001) to S.D. Park., M.K., and S.D.P. are supported by a BK21 Research Fellowship from the Korea Ministry of Education.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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