TY - GEN
T1 - Femtosecond optical injection of intact plant cells using a reconfigurable platform
AU - Mitchell, Claire A.
AU - Kalies, Stefan
AU - Cizmar, Tomas
AU - Bellini, Nicola
AU - Kubasik-Thayil, Anisha
AU - Heisterkamp, Alexander
AU - Torrance, Lesley
AU - Roberts, Alison G.
AU - Gunn-Moore, Frank J.
AU - Dholakia, Kishan
PY - 2014
Y1 - 2014
N2 - The use of ultrashort-pulsed lasers for molecule delivery and transfection has proved to be a non-invasive and highly efficient technique for a wide range of mammalian cells. This present study investigates the effectiveness of femtosecond photoporation in plant cells, a hard-to-manipulate yet agriculturally relevant cell type, specifically suspension tobacco BY-2 cells. Both spatial and temporal shaping of the light field is employed to optimise the delivery of membrane impermeable molecules into plant cells using a reconfigurable optical system designed to be able to switch easily between different spatial modes and pulse durations. The use of a propagation invariant Bessel beam was found to increase the number of cells that could be viably optoinjected, when compared to the use of a Gaussian beam. Photoporation with a laser producing sub-12 fs pulses, coupled with a dispersion compensation system to retain the pulse duration at focus, reduced the power required for efficient optical injection by 1.5-1.8 times when compared to a photoporation with a 140 fs laser output.
AB - The use of ultrashort-pulsed lasers for molecule delivery and transfection has proved to be a non-invasive and highly efficient technique for a wide range of mammalian cells. This present study investigates the effectiveness of femtosecond photoporation in plant cells, a hard-to-manipulate yet agriculturally relevant cell type, specifically suspension tobacco BY-2 cells. Both spatial and temporal shaping of the light field is employed to optimise the delivery of membrane impermeable molecules into plant cells using a reconfigurable optical system designed to be able to switch easily between different spatial modes and pulse durations. The use of a propagation invariant Bessel beam was found to increase the number of cells that could be viably optoinjected, when compared to the use of a Gaussian beam. Photoporation with a laser producing sub-12 fs pulses, coupled with a dispersion compensation system to retain the pulse duration at focus, reduced the power required for efficient optical injection by 1.5-1.8 times when compared to a photoporation with a 140 fs laser output.
UR - http://www.scopus.com/inward/record.url?scp=84900477481&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84900477481&partnerID=8YFLogxK
U2 - 10.1117/12.2037784
DO - 10.1117/12.2037784
M3 - Conference contribution
AN - SCOPUS:84900477481
SN - 9780819498854
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Frontiers in Ultrafast Optics
PB - SPIE
T2 - Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV
Y2 - 2 February 2014 through 5 February 2014
ER -