Failure of setting of mineral trioxide aggregate in the presence of fetal bovine serum and its prevention

Yuran Kim, Sinyoung Kim, Yoo Seok Shin, Il Young Jung, Seung Jong Lee

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)


Introduction: The purpose of this study was to examine the effect of fetal bovine serum (FBS) on the setting of 2 white mineral trioxide aggregates (MTAs) and to determine if calcium chloride (CaCl2) alters the setting behavior of both MTAs under this condition. Methods: Two types of MTA cements (ProRoot MTA [Dentsply Tulsa Dental, Tulsa, OK] and MTA-Angelus [Angelus Dental Solutions, Londrina, Brazil]) were mixed with either distilled water (DW) or 10% CaCl2 solution. Mixed MTA was placed in a polyethylene mold, and each mold was then placed in saline or FBS-soaked Oasis. After storing for 4 days, the MTA samples were removed from the molds, and the microhardness was independently measured at 3 different levels: 0.5 mm, 2 mm, and 4 mm away from the bottom in contact with the immersion solution. Results: None of the samples mixed with DW and exposed to FBS for 4 days had set at the 0.5-mm and 2-mm levels. CaCl2 was effective in reducing (the 0.5-mm level) or eliminating (the 2- and 4-mm levels) the adverse effect of FBS. The groups with short-term exposure to FBS had the same surface microhardness as the groups stored in saline-soaked Oasis. Conclusions: MTA did not set when the mixed MTA was exposed to FBS for 4 days. In these cases, 10% CaCl2 helped the setting of MTA, and the effect was more pronounced in ProRoot MTA than in MTA-Angelus. In contrast, short-term exposure to FBS had minimal or no detrimental effects on the setting behaviors of both MTAs.

Original languageEnglish
Pages (from-to)536-540
Number of pages5
JournalJournal of endodontics
Issue number4
Publication statusPublished - 2012 Apr

All Science Journal Classification (ASJC) codes

  • General Dentistry


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