A one-step grafting method of NHS-ester group is described for the immobilization of enzymes onto a silica surface at room temperature. Pure NHS-ester functionalized methallylsilane was prepared as a grafting reagent and showed remarkable grafting efficiency with acid catalysts at room temperature. As a result, mesoporous silica balls bearing a covalently immobilized enzyme could be obtained with a high loading rate compared to conventional multistep grafting methods. This one-step procedure offers the advantages of precise measurement of the high loading efficiency of the NHS-ester group, and the exclusive formation of only the NHS-ester group, which reduces the presence of unreacted functionalities. A hard day's graft: NHS-ester-functionalized methallylsilanes showed remarkable grafting efficiencies with an acid catalyst. Mesoporous silica balls bearing covalently immobilized enzyme were obtained with high loading compared with conventional multistep grafting methods.
All Science Journal Classification (ASJC) codes
- Organic Chemistry