Fabrication of enzyme reactor utilizing magnetic porous polymer membrane for screening D-Amino acid oxidase inhibitors

Jun Fang Jiang, Juan Qiao, Xiao Yu Mu, Myeong Hee Moon, Li Qi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


In this work, a unique D-amino acid oxidase reactor for enhanced enzymolysis efficiency is presented. A kind of magnetic polymer matrices, composed of iron oxide nanoparticles and porous polymer membrane (poly styrene-co-maleic anhydride), was prepared. With covalent bonding D-Amino acid oxidase on the surface of the matrices and characterization of scanning electron microscope and vibrating sample magnetometer, it demonstrated that the membrane enzyme reactor was successfully constructed. The enzymolysis efficiency of the enzyme reactor was evaluated and the apparent Michaelis-Menten constants of D-Amino acid oxidase were determined (Km was 1.10 mM, Vmax was 23.8 mM min−1) by a chiral ligand exchange capillary electrophoresis protocol with methionine as the substrate. The results indicated that the enzyme reactor could exhibit good stability and excellent reusability. Importantly, because the enzyme and the substrate could be confined into the pores of the matrices, the enzyme reactor displayed the improved enzymolysis efficiency due to the confinement effect. Further, the prepared enzyme reactor was applied for D-Amino acid oxidase inhibitors screening. It has displayed that the proposed protocol could pave a new way for fabrication of novel porous polymer membrane based enzyme reactors to screen enzyme inhibitors.

Original languageEnglish
Pages (from-to)251-257
Number of pages7
Publication statusPublished - 2017 Apr 1

Bibliographical note

Funding Information:
We gratefully acknowledge the financial support from National Natural Science Foundation of China (Grants 21575144, 21375132, 21475137, 21611540335, 21635008, 21621062) and Chinese Academy of Sciences (QYZDJ-SSW-SLH034). M.H. Moon is appreciative of support from the National Research Foundation of Korea (NRF-016K2A9A2A06004726).

Publisher Copyright:
© 2016 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry


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