Eyedrop vaccination induced systemic and mucosal immunity against influenza virus in ferrets

Sangchul Yoon; Eun Do Kim; Min Suk Song; Soo Jung Han; Tae Kwann Park; Kyoung Sub Choi; Young Ki Choi; Kyoung Yul Seo

doi:10.1371/journal.pone.0157634

Eyedrop vaccination induced systemic and mucosal immunity against influenza virus in ferrets

Sangchul Yoon, Eun Do Kim, Min Suk Song, Soo Jung Han, Tae Kwann Park, Kyoung Sub Choi, Young Ki Choi, Kyoung Yul Seo

Department of Ophthalmology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We investigated eyedrop vaccination (EDV) in pre-clinical development for immunological protection against influenza and for potential side effects involving ocular inflammation and the central nervous system (CNS). Live attenuated influenza EDV, CA07 (H1N1), PZ-4 (H1N2) and Uruguay (H3N2), induced both systemic and mucosal virus-specific antibody responses in ferrets. In addition, EDV resulted in a clinically significant protection against viral challenge, and suppression of viral replication in nasal secretion and lung tissue. Regarding safety, we found that administered EDV flow through the tear duct to reach the base of nasal cavity, and thus do not contact the olfactory bulb. All analyses for potential adverse effects due to EDV, including histological and functional examinations, did not reveal significant side effects. On the basis of these findings, we propose that EDV as effective, while being a safe administration route with minimum local side effects, CNS invasion, or visual function disturbance.

Original language	English
Article number	e0157634
Journal	PloS one
Volume	11
Issue number	6
DOIs	https://doi.org/10.1371/journal.pone.0157634
Publication status	Published - 2016 Jun

Bibliographical note

Funding Information:
This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2013M3A9D5072551).

Publisher Copyright:
© 2016 Yoon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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title = "Eyedrop vaccination induced systemic and mucosal immunity against influenza virus in ferrets",

abstract = "We investigated eyedrop vaccination (EDV) in pre-clinical development for immunological protection against influenza and for potential side effects involving ocular inflammation and the central nervous system (CNS). Live attenuated influenza EDV, CA07 (H1N1), PZ-4 (H1N2) and Uruguay (H3N2), induced both systemic and mucosal virus-specific antibody responses in ferrets. In addition, EDV resulted in a clinically significant protection against viral challenge, and suppression of viral replication in nasal secretion and lung tissue. Regarding safety, we found that administered EDV flow through the tear duct to reach the base of nasal cavity, and thus do not contact the olfactory bulb. All analyses for potential adverse effects due to EDV, including histological and functional examinations, did not reveal significant side effects. On the basis of these findings, we propose that EDV as effective, while being a safe administration route with minimum local side effects, CNS invasion, or visual function disturbance.",

author = "Sangchul Yoon and Kim, {Eun Do} and Song, {Min Suk} and Han, {Soo Jung} and Park, {Tae Kwann} and Choi, {Kyoung Sub} and Choi, {Young Ki} and Seo, {Kyoung Yul}",

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AU - Choi, Kyoung Sub

AU - Choi, Young Ki

AU - Seo, Kyoung Yul

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N2 - We investigated eyedrop vaccination (EDV) in pre-clinical development for immunological protection against influenza and for potential side effects involving ocular inflammation and the central nervous system (CNS). Live attenuated influenza EDV, CA07 (H1N1), PZ-4 (H1N2) and Uruguay (H3N2), induced both systemic and mucosal virus-specific antibody responses in ferrets. In addition, EDV resulted in a clinically significant protection against viral challenge, and suppression of viral replication in nasal secretion and lung tissue. Regarding safety, we found that administered EDV flow through the tear duct to reach the base of nasal cavity, and thus do not contact the olfactory bulb. All analyses for potential adverse effects due to EDV, including histological and functional examinations, did not reveal significant side effects. On the basis of these findings, we propose that EDV as effective, while being a safe administration route with minimum local side effects, CNS invasion, or visual function disturbance.

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Eyedrop vaccination induced systemic and mucosal immunity against influenza virus in ferrets

Abstract

Bibliographical note

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UN SDGs

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