Extraordinary Transmission-based Plasmonic Nanoarrays for Axially Super-Resolved Cell Imaging

Jong ryul Choi; Kyujung Kim; Youngjin Oh; Ah Leum Kim; Sook Young Kim; Jeon Soo Shin; Donghyun Kim

doi:10.1002/adom.201300330

Extraordinary Transmission-based Plasmonic Nanoarrays for Axially Super-Resolved Cell Imaging

Jong ryul Choi, Kyujung Kim, Youngjin Oh, Ah Leum Kim, Sook Young Kim, Jeon Soo Shin, Donghyun Kim

Department of Electrical and Electronic Engineering

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

Extraordinary transmission based axial imaging (EOT-AIM) for cell microscopy is reported. EOT-AIM uses linear arrays of nanoapertures, each of which samples target fluorescence up to a preset axial distance from surface, in combination with wide-field microscopy for acquisition of lateral images. Current design of nanoapertures provides EOT-AIM with axial super-resolution that is as small as 20 nm for a depth range of 500 nm. Experiments were performed for the measurement of the axial distribution of ganglioside in mouse macrophage (RAW264.7) cells using FITC-conjugated cholera toxin subunit B. The results were successfully confirmed with conventional confocal and total internal reflection fluorescence microscopy.

Original language	English
Pages (from-to)	48-55
Number of pages	8
Journal	Advanced Optical Materials
Volume	2
Issue number	1
DOIs	https://doi.org/10.1002/adom.201300330
Publication status	Published - 2014 Jan

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics

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10.1002/adom.201300330

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abstract = "Extraordinary transmission based axial imaging (EOT-AIM) for cell microscopy is reported. EOT-AIM uses linear arrays of nanoapertures, each of which samples target fluorescence up to a preset axial distance from surface, in combination with wide-field microscopy for acquisition of lateral images. Current design of nanoapertures provides EOT-AIM with axial super-resolution that is as small as 20 nm for a depth range of 500 nm. Experiments were performed for the measurement of the axial distribution of ganglioside in mouse macrophage (RAW264.7) cells using FITC-conjugated cholera toxin subunit B. The results were successfully confirmed with conventional confocal and total internal reflection fluorescence microscopy.",

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AU - Choi, Jong ryul

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AU - Oh, Youngjin

AU - Kim, Ah Leum

AU - Kim, Sook Young

AU - Shin, Jeon Soo

AU - Kim, Donghyun

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AB - Extraordinary transmission based axial imaging (EOT-AIM) for cell microscopy is reported. EOT-AIM uses linear arrays of nanoapertures, each of which samples target fluorescence up to a preset axial distance from surface, in combination with wide-field microscopy for acquisition of lateral images. Current design of nanoapertures provides EOT-AIM with axial super-resolution that is as small as 20 nm for a depth range of 500 nm. Experiments were performed for the measurement of the axial distribution of ganglioside in mouse macrophage (RAW264.7) cells using FITC-conjugated cholera toxin subunit B. The results were successfully confirmed with conventional confocal and total internal reflection fluorescence microscopy.

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Extraordinary Transmission-based Plasmonic Nanoarrays for Axially Super-Resolved Cell Imaging

Abstract

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