Expression of LRIG1, a Negative Regulator of EGFR, Is Dynamically Altered during Different Stages of Gastric Carcinogenesis

Sungsook Yu, Mijeong Yang, Kyung Min Lim, Yejin Cho, Hyunji Kim, Keunwook Lee, Sang Ho Jeong, Robert J. Coffey, James R. Goldenring, Ki Taek Nam

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9 Citations (Scopus)


Leucine-rich repeats and immunoglobulin-like domains (LRIG)-1 is a transmembrane protein that antagonizes epidermal growth factor receptor signaling in epithelial tissues. LRIG1 is down-regulated in various epithelial cancers, including bladder, breast, and colorectal cancer, suggesting that it functions as a tumor suppressor. However, its role in gastric carcinogenesis is not well understood. Here, we investigated the changes in LRIG1 expression during the stages of gastric cancer. We used a DMP-777–induced spasmolytic polypeptide-expressing metaplasia mouse model and a tissue array of human gastric cancer lesions. The effects of LRIG1 knockdown were also assessed using the human gastric cancer cell line SNU638 in a xenograft model. LRIG1 expression varied over the course of gastric carcinogenesis, increasing in spasmolytic polypeptide-expressing metaplasia lesions but disappearing in intestinal metaplasia and cancer lesions, and the increase was concurrent with the up-regulation of epidermal growth factor receptor. In addition, LRIG1 knockdown promoted the tumorigenic potential in vitro, which was manifested as increased proliferation, invasiveness, and migration as well as increased tumor size in vivo in the xenograft model. Furthermore, LRIG1 expression was determined to be a positive prognostic biomarker for the survival of gastric cancer patients. Collectively, our findings indicate that LRIG1 expression is closely related wto gastric carcinogenesis and may play a vital role as a tumor suppressor through the modulation of epidermal growth factor receptor activity.

Original languageEnglish
Pages (from-to)2912-2923
Number of pages12
JournalAmerican Journal of Pathology
Issue number12
Publication statusPublished - 2018 Dec

Bibliographical note

Funding Information:
Supported by National Research Foundation (NRF; funded by the Korean government) Korea Mouse Phenotyping Project grants NRF-2016M3A9D5A01952416 and 2013M3A9D5072551, The Ministry of Science, ICT and Future Planning grants NRF-2017R1A2B2009850 and NRF-2017M3A9F3041234, and Ministry of Science and ICT grant 2018R1A5A2025286; Yonsei University College of Medicine faculty research grant 6-2015-0163; the Yonsei University Brain Korea 21 PLUS Project for Medical Science (K.T.N.); and Ministry of Education, Science and Technology NRF Basic Science Research Program grant NRF-2015R1C1A2A01053575 (S.Y.). J.R.G. is supported by US Department of Veterans Affairs Merit Review Award grant I01BX000930 and NIH grant RO1 DK071590.

Publisher Copyright:
© 2018 American Society for Investigative Pathology

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine


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