Expression of a novel tobacco gene, NgCDM1, is preferentially associated with pathogen-induced cell death

Mi Chung Suh; Sang Keun Oh; Young Cheol Kim; Hyun Sook Pai; Doil Choi

doi:10.1016/S0885-5765(03)00049-3

Expression of a novel tobacco gene, NgCDM1, is preferentially associated with pathogen-induced cell death

Mi Chung Suh, Sang Keun Oh, Young Cheol Kim, Hyun Sook Pai, Doil Choi

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

The nucleotide sequence for NgCDM1 was deposited in GenBank database under the accession number AF208022. Incompatible plant-pathogen interactions result in rapid cell death known as the hypersensitive response (HR) and activation of host defense-related genes. To understand cellular mechanisms controlling HR against pathogen attack, we previously isolated a pool of tobacco genes that are induced or repressed by infection of TMV. In this study, expression of a TMV-induced gene, NgCDM1, which has low sequence homology to known genes was investigated, NgCDM1 mRNA accumulated not only during HR by inoculation of the avirulent pathogen Pseudomonas syringae pv. syringae 61, but also in necrotic cell death caused by infiltration of the virulent pathogen P. s. pv. tabaci. However, induction of the NgCDM1 transcripts was not triggered by cell death caused by senescence or freezing stress. Expression of NgCDM1 mRNA was independent of the plant defense signaling pathways induced by salicylic acid, methyl jasmonate, or H₂O₂ and also not associated with systemic acquired resistance, Immunoblot analysis revealed that NgCDM1 protein coincidently accumulated with induction of NgCDM1 transcript and was localized primarily in the vicinity of HR-lesion. These results indicate that expression of NgCDM1 gene is preferentially associated with pathogen-induced cell death.

Original language	English
Pages (from-to)	227-235
Number of pages	9
Journal	Physiological and Molecular Plant Pathology
Volume	62
Issue number	4
DOIs	https://doi.org/10.1016/S0885-5765(03)00049-3
Publication status	Published - 2003 Apr 1

Bibliographical note

Funding Information:
The authors would like to thank Sang Jik Lee and So Young Yi for technical assistance. Dr Tai-Jin Choi at Pu-Kyung University was responsible for generation of antibodies against the NgCDM1 protein. Dr Sanghyeob Lee provided critical reading of the manuscript. This research was supported by grants from Plant Diversity Research Center and Crop Functional Genomics Center of 21st Century Frontier Research Program and in part by PMGBRC funded by MOST of Korean government.

All Science Journal Classification (ASJC) codes

Genetics
Plant Science

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10.1016/S0885-5765(03)00049-3

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title = "Expression of a novel tobacco gene, NgCDM1, is preferentially associated with pathogen-induced cell death",

abstract = "The nucleotide sequence for NgCDM1 was deposited in GenBank database under the accession number AF208022. Incompatible plant-pathogen interactions result in rapid cell death known as the hypersensitive response (HR) and activation of host defense-related genes. To understand cellular mechanisms controlling HR against pathogen attack, we previously isolated a pool of tobacco genes that are induced or repressed by infection of TMV. In this study, expression of a TMV-induced gene, NgCDM1, which has low sequence homology to known genes was investigated, NgCDM1 mRNA accumulated not only during HR by inoculation of the avirulent pathogen Pseudomonas syringae pv. syringae 61, but also in necrotic cell death caused by infiltration of the virulent pathogen P. s. pv. tabaci. However, induction of the NgCDM1 transcripts was not triggered by cell death caused by senescence or freezing stress. Expression of NgCDM1 mRNA was independent of the plant defense signaling pathways induced by salicylic acid, methyl jasmonate, or H2O2 and also not associated with systemic acquired resistance, Immunoblot analysis revealed that NgCDM1 protein coincidently accumulated with induction of NgCDM1 transcript and was localized primarily in the vicinity of HR-lesion. These results indicate that expression of NgCDM1 gene is preferentially associated with pathogen-induced cell death.",

author = "Suh, {Mi Chung} and Oh, {Sang Keun} and Kim, {Young Cheol} and Pai, {Hyun Sook} and Doil Choi",

note = "Funding Information: The authors would like to thank Sang Jik Lee and So Young Yi for technical assistance. Dr Tai-Jin Choi at Pu-Kyung University was responsible for generation of antibodies against the NgCDM1 protein. Dr Sanghyeob Lee provided critical reading of the manuscript. This research was supported by grants from Plant Diversity Research Center and Crop Functional Genomics Center of 21st Century Frontier Research Program and in part by PMGBRC funded by MOST of Korean government.",

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AU - Oh, Sang Keun

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AU - Pai, Hyun Sook

AU - Choi, Doil

N1 - Funding Information: The authors would like to thank Sang Jik Lee and So Young Yi for technical assistance. Dr Tai-Jin Choi at Pu-Kyung University was responsible for generation of antibodies against the NgCDM1 protein. Dr Sanghyeob Lee provided critical reading of the manuscript. This research was supported by grants from Plant Diversity Research Center and Crop Functional Genomics Center of 21st Century Frontier Research Program and in part by PMGBRC funded by MOST of Korean government.

PY - 2003/4/1

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N2 - The nucleotide sequence for NgCDM1 was deposited in GenBank database under the accession number AF208022. Incompatible plant-pathogen interactions result in rapid cell death known as the hypersensitive response (HR) and activation of host defense-related genes. To understand cellular mechanisms controlling HR against pathogen attack, we previously isolated a pool of tobacco genes that are induced or repressed by infection of TMV. In this study, expression of a TMV-induced gene, NgCDM1, which has low sequence homology to known genes was investigated, NgCDM1 mRNA accumulated not only during HR by inoculation of the avirulent pathogen Pseudomonas syringae pv. syringae 61, but also in necrotic cell death caused by infiltration of the virulent pathogen P. s. pv. tabaci. However, induction of the NgCDM1 transcripts was not triggered by cell death caused by senescence or freezing stress. Expression of NgCDM1 mRNA was independent of the plant defense signaling pathways induced by salicylic acid, methyl jasmonate, or H2O2 and also not associated with systemic acquired resistance, Immunoblot analysis revealed that NgCDM1 protein coincidently accumulated with induction of NgCDM1 transcript and was localized primarily in the vicinity of HR-lesion. These results indicate that expression of NgCDM1 gene is preferentially associated with pathogen-induced cell death.

AB - The nucleotide sequence for NgCDM1 was deposited in GenBank database under the accession number AF208022. Incompatible plant-pathogen interactions result in rapid cell death known as the hypersensitive response (HR) and activation of host defense-related genes. To understand cellular mechanisms controlling HR against pathogen attack, we previously isolated a pool of tobacco genes that are induced or repressed by infection of TMV. In this study, expression of a TMV-induced gene, NgCDM1, which has low sequence homology to known genes was investigated, NgCDM1 mRNA accumulated not only during HR by inoculation of the avirulent pathogen Pseudomonas syringae pv. syringae 61, but also in necrotic cell death caused by infiltration of the virulent pathogen P. s. pv. tabaci. However, induction of the NgCDM1 transcripts was not triggered by cell death caused by senescence or freezing stress. Expression of NgCDM1 mRNA was independent of the plant defense signaling pathways induced by salicylic acid, methyl jasmonate, or H2O2 and also not associated with systemic acquired resistance, Immunoblot analysis revealed that NgCDM1 protein coincidently accumulated with induction of NgCDM1 transcript and was localized primarily in the vicinity of HR-lesion. These results indicate that expression of NgCDM1 gene is preferentially associated with pathogen-induced cell death.

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Expression of a novel tobacco gene, NgCDM1, is preferentially associated with pathogen-induced cell death

Abstract

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