Expanding the genetic code of Mus musculus

Songmi Han; Aerin Yang; Soonjang Lee; Han Woong Lee; Chan Bae Park; Hee Sung Park

doi:10.1038/ncomms14568

Expanding the genetic code of Mus musculus

Songmi Han, Aerin Yang, Soonjang Lee, Han Woong Lee, Chan Bae Park, Hee Sung Park

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including Ne-acetyl-lysine. Stable integration of transgenes encoding an engineered N^ϵ-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNAPyl pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal and spatial control of protein acetylation in various organs of the transgenic mouse using a recombinant green fluorescent protein (GFPuv) as a model protein. This strategy will provide a powerful tool for systematic in vivo study of cellular proteins in the most commonly used mammalian model organism for human physiology and disease.

Original language	English
Article number	14568
Journal	Nature communications
Volume	8
DOIs	https://doi.org/10.1038/ncomms14568
Publication status	Published - 2017 Feb 21

Bibliographical note

Funding Information:
This work was supported by grants from the National Research Foundation of Korea (KAIST Systems Healthcare, 2011-0020322, and 2014M3A6A4075060) to H.-S.P., and the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Science, ICT & Future Planning (2014M3A6A8066439) and Ministry of Food and Drug Safety (14182MFDS978) awarded to C.B.P.

Publisher Copyright:
© The Author(s) 2017.

All Science Journal Classification (ASJC) codes

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/ncomms14568

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abstract = "Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including Ne-acetyl-lysine. Stable integration of transgenes encoding an engineered Nϵ-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNAPyl pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal and spatial control of protein acetylation in various organs of the transgenic mouse using a recombinant green fluorescent protein (GFPuv) as a model protein. This strategy will provide a powerful tool for systematic in vivo study of cellular proteins in the most commonly used mammalian model organism for human physiology and disease.",

author = "Songmi Han and Aerin Yang and Soonjang Lee and Lee, {Han Woong} and Park, {Chan Bae} and Park, {Hee Sung}",

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AU - Park, Hee Sung

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Expanding the genetic code of Mus musculus

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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