Evaluation of urinary nucleosides in breast cancer patients before and after tumor removal

Sung Hee Cho; Man Ho Choi; Won Yong Lee; Bong Chul Chung

doi:10.1016/j.clinbiochem.2008.12.026

Evaluation of urinary nucleosides in breast cancer patients before and after tumor removal

Sung Hee Cho, Man Ho Choi, Won Yong Lee, Bong Chul Chung

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

Objective: Evaluation of altered urinary nucleosides before and after tumor removal of breast cancer (BCa). Design and methods: Targeted metabolite profiling of 14 urinary nucleosides was conducted with both pre- and post-operative female patients with BCa (n = 150, age: 46.6 ± 7.7 years), and female controls (n = 150, age: 46.8 ± 7.7 years) by liquid chromatography-tandem mass spectrometry coupled to on-line extraction. Results: Levels of modified nucleosides (5-hydroxymethyl-2′-deoxyuridine, P < 0.001; 8-hydroxy-2′-deoxyguanosine, P < 0.001; 1-methyladenosine, P < 0.02; N²,N²-dimethylguanosine, P < 0.001) were significantly higher in pre-operative patients than in both normal controls and post-operative patients. Conclusions: This approach could be used to further understand the pathogenesis of BCa as well as to evaluate the effects of medical treatment.

Original language	English
Pages (from-to)	540-543
Number of pages	4
Journal	Clinical Biochemistry
Volume	42
Issue number	6
DOIs	https://doi.org/10.1016/j.clinbiochem.2008.12.026
Publication status	Published - 2009 Apr

Bibliographical note

Funding Information:
This work was supported by an intramural grant from the Korean Institute of Science and Technology (KIST), and by grants from the National R&D Program of the Korean Ministry of Education, Science and Technology (MEST) and the Korean Science and Engineering Foundation (KOSEF).

All Science Journal Classification (ASJC) codes

Clinical Biochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.clinbiochem.2008.12.026

Cite this

@article{3ff1d69492664661b20bc36d09c4ea94,

title = "Evaluation of urinary nucleosides in breast cancer patients before and after tumor removal",

abstract = "Objective: Evaluation of altered urinary nucleosides before and after tumor removal of breast cancer (BCa). Design and methods: Targeted metabolite profiling of 14 urinary nucleosides was conducted with both pre- and post-operative female patients with BCa (n = 150, age: 46.6 ± 7.7 years), and female controls (n = 150, age: 46.8 ± 7.7 years) by liquid chromatography-tandem mass spectrometry coupled to on-line extraction. Results: Levels of modified nucleosides (5-hydroxymethyl-2′-deoxyuridine, P < 0.001; 8-hydroxy-2′-deoxyguanosine, P < 0.001; 1-methyladenosine, P < 0.02; N2,N2-dimethylguanosine, P < 0.001) were significantly higher in pre-operative patients than in both normal controls and post-operative patients. Conclusions: This approach could be used to further understand the pathogenesis of BCa as well as to evaluate the effects of medical treatment.",

author = "Cho, {Sung Hee} and Choi, {Man Ho} and Lee, {Won Yong} and Chung, {Bong Chul}",

note = "Funding Information: This work was supported by an intramural grant from the Korean Institute of Science and Technology (KIST), and by grants from the National R&D Program of the Korean Ministry of Education, Science and Technology (MEST) and the Korean Science and Engineering Foundation (KOSEF). ",

year = "2009",

month = apr,

doi = "10.1016/j.clinbiochem.2008.12.026",

language = "English",

volume = "42",

pages = "540--543",

journal = "Clinical Biochemistry",

issn = "0009-9120",

publisher = "Elsevier Inc.",

number = "6",

}

TY - JOUR

T1 - Evaluation of urinary nucleosides in breast cancer patients before and after tumor removal

AU - Cho, Sung Hee

AU - Choi, Man Ho

AU - Lee, Won Yong

AU - Chung, Bong Chul

N1 - Funding Information: This work was supported by an intramural grant from the Korean Institute of Science and Technology (KIST), and by grants from the National R&D Program of the Korean Ministry of Education, Science and Technology (MEST) and the Korean Science and Engineering Foundation (KOSEF).

PY - 2009/4

Y1 - 2009/4

N2 - Objective: Evaluation of altered urinary nucleosides before and after tumor removal of breast cancer (BCa). Design and methods: Targeted metabolite profiling of 14 urinary nucleosides was conducted with both pre- and post-operative female patients with BCa (n = 150, age: 46.6 ± 7.7 years), and female controls (n = 150, age: 46.8 ± 7.7 years) by liquid chromatography-tandem mass spectrometry coupled to on-line extraction. Results: Levels of modified nucleosides (5-hydroxymethyl-2′-deoxyuridine, P < 0.001; 8-hydroxy-2′-deoxyguanosine, P < 0.001; 1-methyladenosine, P < 0.02; N2,N2-dimethylguanosine, P < 0.001) were significantly higher in pre-operative patients than in both normal controls and post-operative patients. Conclusions: This approach could be used to further understand the pathogenesis of BCa as well as to evaluate the effects of medical treatment.

AB - Objective: Evaluation of altered urinary nucleosides before and after tumor removal of breast cancer (BCa). Design and methods: Targeted metabolite profiling of 14 urinary nucleosides was conducted with both pre- and post-operative female patients with BCa (n = 150, age: 46.6 ± 7.7 years), and female controls (n = 150, age: 46.8 ± 7.7 years) by liquid chromatography-tandem mass spectrometry coupled to on-line extraction. Results: Levels of modified nucleosides (5-hydroxymethyl-2′-deoxyuridine, P < 0.001; 8-hydroxy-2′-deoxyguanosine, P < 0.001; 1-methyladenosine, P < 0.02; N2,N2-dimethylguanosine, P < 0.001) were significantly higher in pre-operative patients than in both normal controls and post-operative patients. Conclusions: This approach could be used to further understand the pathogenesis of BCa as well as to evaluate the effects of medical treatment.

UR - http://www.scopus.com/inward/record.url?scp=61849178717&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61849178717&partnerID=8YFLogxK

U2 - 10.1016/j.clinbiochem.2008.12.026

DO - 10.1016/j.clinbiochem.2008.12.026

M3 - Article

C2 - 19186175

AN - SCOPUS:61849178717

SN - 0009-9120

VL - 42

SP - 540

EP - 543

JO - Clinical Biochemistry

JF - Clinical Biochemistry

IS - 6

ER -

Evaluation of urinary nucleosides in breast cancer patients before and after tumor removal

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this