Abstract
The poly (ethylene glycol)-poly (L-lactide) (PEG-PLGA) film/collagen-hyaluronic acid (HA) composite material was newly developed as a tissue adhesion preventive membrane. In this study, the effects of the composite matrix on the cell adhesion and proliferation were evaluated. Fibroblasts attached better on the collagen-HA matrix than on Interceed™ or PEG-PLGA film. PEG-PLGA showed very low cell adhesive property. SEM observations revealed that the fibroblasts attached evenly on the collagen-HA matrix due to its high water uptake ability and hydrophilicity. In confocal microscopic observation, it was shown that actin microfilaments of fibroblasts were further polymerized on the collagen-HA membrane compared to those on the PEG-PLGA membrane and Interceed™. The proliferation test showed that proliferation of fibroblasts was enhanced by collagen-HA matrix. However, very few attached cells were found on the surface of the PEG-PLGA film after 7-day incubation. Therefore, the bioresorbable composite material containing collagen-HA matrix side, which was designed for the damaged layer of peritoneum and PEG-PLGA film side for preventing tissue adhesion, could be proposed as a novel adhesion barrier membrane.
| Original language | English |
|---|---|
| Pages (from-to) | 3255-3260 |
| Number of pages | 6 |
| Journal | Materials Science Forum |
| Volume | 426-432 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2003 |
| Event | Thermec 2003 Processing and Manufacturing of Advanced Materials - Madrid, Spain Duration: 2003 Jul 7 → 2003 Jul 11 |
All Science Journal Classification (ASJC) codes
- General Materials Science
- Condensed Matter Physics
- Mechanics of Materials
- Mechanical Engineering