TY - JOUR
T1 - Evaluation of the safety of xenon/bandpass light in vitrectomy using the A2E-laden RPE model
AU - Yanagi, Yasuo
AU - Iriyama, Aya
AU - Jang, Woo Dong
AU - Kadonosono, Kazuaki
PY - 2007/5
Y1 - 2007/5
N2 - Background: The purpose of the study was to investigate the brightness of the xenon/bandpass light in vitrectomy and assess its phototoxic effects using A2E-laden retinal pigment epithelial (RPE) cells. Methods: The total luminous flux and spectral irradiance of 20- and 25-gauge endoilluminators connected to xenon lamps were measured and compared to those of 20- and 25-gauge endoilluminators connected to a halogen lamp. In vitro, A2E-laden cells were evenly exposed to xenon/ bandpass light for 5 to 30 min positioned at 1 cm and 2 cm for a standard light probe and an implantable "chandelier" light probe, respectively, above the cells, and the cell viability was assessed using WST-1 assay. The cell viability was compared with cells exposed to 30 min of halogen light projected through a 20-gauge endoilluminator. Results: The maximal total luminous flux of xenon/bandpass light emitted through the 20-gauge endoilluminator was 2.8 times higher than that of the halogen light. The total luminous flux of the 25-gauge endoilluminators was 0.6-1.1 times greater than the 20-gauge endoilluminators connected to the halogen light. The viability of the A2E-laden cells after exposure to the xenon/bandpass light was no different than that of the cells exposed to the halogen light when the total luminous flux of these lights was at the same level. Xenon/ bandpass light from an implantable "chandelier" light probe induced A2E-mediated RPE damage to a similar extent as that of the halogen light through a 20-gauge endoilluminator. Conclusions: A2E-mediated phototoxicity of xenon/bandpass light is comparable to that of halogen light.
AB - Background: The purpose of the study was to investigate the brightness of the xenon/bandpass light in vitrectomy and assess its phototoxic effects using A2E-laden retinal pigment epithelial (RPE) cells. Methods: The total luminous flux and spectral irradiance of 20- and 25-gauge endoilluminators connected to xenon lamps were measured and compared to those of 20- and 25-gauge endoilluminators connected to a halogen lamp. In vitro, A2E-laden cells were evenly exposed to xenon/ bandpass light for 5 to 30 min positioned at 1 cm and 2 cm for a standard light probe and an implantable "chandelier" light probe, respectively, above the cells, and the cell viability was assessed using WST-1 assay. The cell viability was compared with cells exposed to 30 min of halogen light projected through a 20-gauge endoilluminator. Results: The maximal total luminous flux of xenon/bandpass light emitted through the 20-gauge endoilluminator was 2.8 times higher than that of the halogen light. The total luminous flux of the 25-gauge endoilluminators was 0.6-1.1 times greater than the 20-gauge endoilluminators connected to the halogen light. The viability of the A2E-laden cells after exposure to the xenon/bandpass light was no different than that of the cells exposed to the halogen light when the total luminous flux of these lights was at the same level. Xenon/ bandpass light from an implantable "chandelier" light probe induced A2E-mediated RPE damage to a similar extent as that of the halogen light through a 20-gauge endoilluminator. Conclusions: A2E-mediated phototoxicity of xenon/bandpass light is comparable to that of halogen light.
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U2 - 10.1007/s00417-006-0428-x
DO - 10.1007/s00417-006-0428-x
M3 - Article
C2 - 17043808
AN - SCOPUS:34250719381
SN - 0065-6100
VL - 245
SP - 677
EP - 681
JO - Albrecht von Graefes Archiv für Klinische und Experimentelle Ophthalmologie
JF - Albrecht von Graefes Archiv für Klinische und Experimentelle Ophthalmologie
IS - 5
ER -