Evaluation of the international society for cutaneous lymphoma algorithm for the diagnosis of early mycosis fungoides

Hyang Joo Ryu; Sun Il Kim; Hyung Ook Jang; Se Hoon Kim; Sang Ho Oh; Sujin Park; Sang Kyum Kim

doi:10.3390/cells10102758

Evaluation of the international society for cutaneous lymphoma algorithm for the diagnosis of early mycosis fungoides

Hyang Joo Ryu, Sun Il Kim, Hyung Ook Jang, Se Hoon Kim, Sang Ho Oh, Sujin Park, Sang Kyum Kim

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The International Society for Cutaneous Lymphoma (ISCL) proposes a diagnostic algorithm for early mycosis fungoides (MF) that includes clinical, histological, immunophenotypical, and molecular criteria. Here, we analyzed the immunologic markers and features of T-cell clonality in 38 early MF cases and 22 non-MF cases to validate the ISCL algorithm. We found that CD5 and CD7 expression differed significantly between early MF and non-MF cases, with epidermal discordance of CD7 expression more frequently identified in early MF. Notably, increasing the cut-off value for CD7 expression from 10% to 22.5% improved its sensitivity. Furthermore, TCR-γ and β chain rearrangements were more frequently detected in early MF than in non-MF cases. Based on these findings, we propose CD5 and CD7 deficiency as mandatory immunopathologic criteria and PCR-based testing for TCR-γ and β chains as required molecular/biologic criteria to improve the efficiency of early MF diagnosis using the ISCL algorithm.

Original language	English
Article number	2758
Journal	Cells
Volume	10
Issue number	10
DOIs	https://doi.org/10.3390/cells10102758
Publication status	Published - 2021 Oct

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Funding Information:
Funding: This research was funded by the Basic Science Research Program through the National Research Foundation of Korea (NRF), grant number 2020R1F1A1076774 (S.-K.K.).

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

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@article{54d97ada91fd46c4800ac033d1411364,

title = "Evaluation of the international society for cutaneous lymphoma algorithm for the diagnosis of early mycosis fungoides",

abstract = "The International Society for Cutaneous Lymphoma (ISCL) proposes a diagnostic algorithm for early mycosis fungoides (MF) that includes clinical, histological, immunophenotypical, and molecular criteria. Here, we analyzed the immunologic markers and features of T-cell clonality in 38 early MF cases and 22 non-MF cases to validate the ISCL algorithm. We found that CD5 and CD7 expression differed significantly between early MF and non-MF cases, with epidermal discordance of CD7 expression more frequently identified in early MF. Notably, increasing the cut-off value for CD7 expression from 10% to 22.5% improved its sensitivity. Furthermore, TCR-γ and β chain rearrangements were more frequently detected in early MF than in non-MF cases. Based on these findings, we propose CD5 and CD7 deficiency as mandatory immunopathologic criteria and PCR-based testing for TCR-γ and β chains as required molecular/biologic criteria to improve the efficiency of early MF diagnosis using the ISCL algorithm.",

author = "Ryu, {Hyang Joo} and Kim, {Sun Il} and Jang, {Hyung Ook} and Kim, {Se Hoon} and Oh, {Sang Ho} and Sujin Park and Kim, {Sang Kyum}",

note = "Funding Information: Funding: This research was funded by the Basic Science Research Program through the National Research Foundation of Korea (NRF), grant number 2020R1F1A1076774 (S.-K.K.). Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

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doi = "10.3390/cells10102758",

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AU - Ryu, Hyang Joo

AU - Kim, Sun Il

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AU - Kim, Se Hoon

AU - Oh, Sang Ho

AU - Park, Sujin

AU - Kim, Sang Kyum

N1 - Funding Information: Funding: This research was funded by the Basic Science Research Program through the National Research Foundation of Korea (NRF), grant number 2020R1F1A1076774 (S.-K.K.). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/10

Y1 - 2021/10

N2 - The International Society for Cutaneous Lymphoma (ISCL) proposes a diagnostic algorithm for early mycosis fungoides (MF) that includes clinical, histological, immunophenotypical, and molecular criteria. Here, we analyzed the immunologic markers and features of T-cell clonality in 38 early MF cases and 22 non-MF cases to validate the ISCL algorithm. We found that CD5 and CD7 expression differed significantly between early MF and non-MF cases, with epidermal discordance of CD7 expression more frequently identified in early MF. Notably, increasing the cut-off value for CD7 expression from 10% to 22.5% improved its sensitivity. Furthermore, TCR-γ and β chain rearrangements were more frequently detected in early MF than in non-MF cases. Based on these findings, we propose CD5 and CD7 deficiency as mandatory immunopathologic criteria and PCR-based testing for TCR-γ and β chains as required molecular/biologic criteria to improve the efficiency of early MF diagnosis using the ISCL algorithm.

AB - The International Society for Cutaneous Lymphoma (ISCL) proposes a diagnostic algorithm for early mycosis fungoides (MF) that includes clinical, histological, immunophenotypical, and molecular criteria. Here, we analyzed the immunologic markers and features of T-cell clonality in 38 early MF cases and 22 non-MF cases to validate the ISCL algorithm. We found that CD5 and CD7 expression differed significantly between early MF and non-MF cases, with epidermal discordance of CD7 expression more frequently identified in early MF. Notably, increasing the cut-off value for CD7 expression from 10% to 22.5% improved its sensitivity. Furthermore, TCR-γ and β chain rearrangements were more frequently detected in early MF than in non-MF cases. Based on these findings, we propose CD5 and CD7 deficiency as mandatory immunopathologic criteria and PCR-based testing for TCR-γ and β chains as required molecular/biologic criteria to improve the efficiency of early MF diagnosis using the ISCL algorithm.

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Evaluation of the international society for cutaneous lymphoma algorithm for the diagnosis of early mycosis fungoides

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