Evaluation of TBMDR® and XDRA® for the detection of multidrug resistant and pre-extensively drug resistant tuberculosis

Eunjin Cho, Su jin Lee, Jiyoung Lim, Dong Sik Kim, Namil Kim, Han Oh Park, Ji im Lee, Eunsoon Son, Sang Nae Cho, Wah Wah Aung, Jong Seok Lee

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

This study evaluated the diagnostic performance of the AccuPower® TB&MDR Real-Time PCR (TBMDR®) and AccuPower® XDR-TB Real-Time PCR Kit-A (XDRA®) to detect multidrug-resistant (MDR-TB) and pre-extensively drug-resistant tuberculosis (pre-XDR-TB) in comparison with phenotypic drug susceptibility testing (DST) using MGIT 960 on 234 clinical Mycobacterium tuberculosis isolates. Discrepant results were confirmed by direct-sequencing. Sensitivity and specificity of TBMDR and XDRA for cultured isolates were 81.2% and 95.8% for isoniazid (INH) resistance, 95.7% and 95.7% for rifampicin (RIF) resistance, 84.1% and 99.1% for fluoroquinolone (FQ) resistance, and 67.4% and 100% for second-line injectables resistance. The sensitivities of each drug were equivalent to other molecular DST methods. High concordance was observed when compared to direct-sequencing. We also found that TBMDR and XDRA assays can detect INH, RIF and FQ resistance in isolates with low level resistance-associated mutations which were missed by phenotypic DST. Our study showed TBMDR and XDRA assays could be the useful tools to detect MDR-TB and pre-XDR-TB.

Original languageEnglish
Article number100303
JournalJournal of Clinical Tuberculosis and Other Mycobacterial Diseases
Volume27
DOIs
Publication statusPublished - 2022 May

Bibliographical note

Publisher Copyright:
© 2022

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine
  • Microbiology (medical)
  • Infectious Diseases

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