TY - JOUR
T1 - Evaluation of phenotypic screening methods for detecting plasmid-mediated AmpC β-lactamases-producing isolates of Escherichia coli and Klebsiella pneumoniae
AU - Lee, Kyungwon
AU - Hong, Seong Geun
AU - Park, Yeon Joon
AU - Lee, Hye Soo
AU - Song, Wonkeun
AU - Jeong, Joseph
AU - Yong, Dongeun
AU - Chong, Yunsop
PY - 2005/12
Y1 - 2005/12
N2 - Detection of plasmid-mediated (P-M) AmpC β-lactamase-producing isolates is considered critical for epidemiologic studies and hospital infection control, but the documents of the Clinical and Laboratory Standards Institute do not contain any recommendation for the phenotypic detection. In this study, phenotypic detection methods, cefoxitin-Hodge test and induction test, were evaluated using cefoxitin-resistant Escherichia coli and Klebsiella pneumoniae isolates. The cefoxitin-Hodge test detected all blaCMY-10, and 97.4% of blaCMY-2 allele-positive isolates, but only 57.3% of bla DHA-1 allele-positive isolates. Induction test with an aztreonam and an amoxicillin-clavulanic acid disk was more sensitive than with cefoxitin disk, which detected 86.6% of blaDHA-1 allele-positive isolates. These phenotypic tests should be useful to screen P-M AmpC β-lactamase-producing E. coli and K. pneumoniae isolates.
AB - Detection of plasmid-mediated (P-M) AmpC β-lactamase-producing isolates is considered critical for epidemiologic studies and hospital infection control, but the documents of the Clinical and Laboratory Standards Institute do not contain any recommendation for the phenotypic detection. In this study, phenotypic detection methods, cefoxitin-Hodge test and induction test, were evaluated using cefoxitin-resistant Escherichia coli and Klebsiella pneumoniae isolates. The cefoxitin-Hodge test detected all blaCMY-10, and 97.4% of blaCMY-2 allele-positive isolates, but only 57.3% of bla DHA-1 allele-positive isolates. Induction test with an aztreonam and an amoxicillin-clavulanic acid disk was more sensitive than with cefoxitin disk, which detected 86.6% of blaDHA-1 allele-positive isolates. These phenotypic tests should be useful to screen P-M AmpC β-lactamase-producing E. coli and K. pneumoniae isolates.
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U2 - 10.1016/j.diagmicrobio.2005.07.004
DO - 10.1016/j.diagmicrobio.2005.07.004
M3 - Article
C2 - 16269222
AN - SCOPUS:29144473484
SN - 0732-8893
VL - 53
SP - 319
EP - 323
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 4
ER -
