Enzymatic esterification of β-methylglucoside with acrylic/ methacrylic acid in organic solvents

Dae Won Park, Seungjoo Haam, Ik Sung Ahn, Tai Gyu Lee, Hae Sung Kim, Woo Sik Kim

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27 Citations (Scopus)


The enzymatic esterifications of β-methylglucoside with acrylic acid/methacrylic acid were carried out using Novozym 435. t-Butanol indicating the highest conversion value was determined as an optimal solvent. The molar ratio (β-methylglucoside:acids) of 1:15 was most favorable to the esterification. The enzyme concentration of 5% (w/v), and the temperature (50°C for β-methylglucoside:acrylic acid, 45°C for β-methylglucoside:methacrylic acid) resulted in the highest final conversion. β-Methylglucoside of 60 gl-1 was found to be most effective in terms of short reaction time as well as product concentrations. Under these conditions, the maximum conversions for the esterification of β-methylglucoside with acrylic acid and β-methylglucoside with methacrylic acid were 59.3% after 12 h and 71.3% after 72 h, respectively. The structural analysis of the products was performed by FT-IR spectroscopy and 1H NMR.

Original languageEnglish
Pages (from-to)151-160
Number of pages10
JournalJournal of Biotechnology
Issue number2
Publication statusPublished - 2004 Jan 22

Bibliographical note

Funding Information:
The authors gratefully acknowledge the financial support of Advanced Environmental Biology Research Center (R11-2003-006).

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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