Entrapment of enzyme-linked magnetic nanoparticles within poly(ethylene glycol) hydrogel microparticles prepared by photopatterning

Sami Park; Yeol Lee; Dae Nyun Kim; Sangphil Park; Eunji Jang; Won Gun Koh

doi:10.1016/j.reactfunctpolym.2009.02.001

Entrapment of enzyme-linked magnetic nanoparticles within poly(ethylene glycol) hydrogel microparticles prepared by photopatterning

Sami Park, Yeol Lee, Dae Nyun Kim, Sangphil Park, Eunji Jang, Won Gun Koh

Department of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

In this study, hydrogel microparticles containing enzyme-linked magnetic nanoparticles (MNPs) were prepared. Peroxidase (POD), a model enzyme, was covalently immobilized on the surface of MNPs using 3-aminopropyltriethoxysilane (APTES), and the resultant POD-linked MNPs were entrapped within various shapes of hydrogel microparticles using photopatterning. Pre-immobilizing POD on the MNP surface made it possible to use hydrogels prepared from high molecular weight PEG without enzyme leaching, which enhanced the reaction rate of entrapped enzymes by reducing resistance to mass transport. Quantitative assays showed a linear correspondence between fluorescence intensity and H₂O₂ concentrations below 15 mM.

Original language	English
Pages (from-to)	293-299
Number of pages	7
Journal	Reactive and Functional Polymers
Volume	69
Issue number	5
DOIs	https://doi.org/10.1016/j.reactfunctpolym.2009.02.001
Publication status	Published - 2009 May

Bibliographical note

Funding Information:
This work was supported by Korea Science and Engineering Foundation (KOSEF) funded by MEST (R11-2007-050-03002-0 and R01-2007-000-11495).

All Science Journal Classification (ASJC) codes

Chemistry(all)
Environmental Chemistry
Biochemistry
Chemical Engineering(all)
Polymers and Plastics
Materials Chemistry

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10.1016/j.reactfunctpolym.2009.02.001

Cite this

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title = "Entrapment of enzyme-linked magnetic nanoparticles within poly(ethylene glycol) hydrogel microparticles prepared by photopatterning",

abstract = "In this study, hydrogel microparticles containing enzyme-linked magnetic nanoparticles (MNPs) were prepared. Peroxidase (POD), a model enzyme, was covalently immobilized on the surface of MNPs using 3-aminopropyltriethoxysilane (APTES), and the resultant POD-linked MNPs were entrapped within various shapes of hydrogel microparticles using photopatterning. Pre-immobilizing POD on the MNP surface made it possible to use hydrogels prepared from high molecular weight PEG without enzyme leaching, which enhanced the reaction rate of entrapped enzymes by reducing resistance to mass transport. Quantitative assays showed a linear correspondence between fluorescence intensity and H2O2 concentrations below 15 mM.",

author = "Sami Park and Yeol Lee and Kim, {Dae Nyun} and Sangphil Park and Eunji Jang and Koh, {Won Gun}",

note = "Funding Information: This work was supported by Korea Science and Engineering Foundation (KOSEF) funded by MEST (R11-2007-050-03002-0 and R01-2007-000-11495).",

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AU - Lee, Yeol

AU - Kim, Dae Nyun

AU - Park, Sangphil

AU - Jang, Eunji

AU - Koh, Won Gun

N1 - Funding Information: This work was supported by Korea Science and Engineering Foundation (KOSEF) funded by MEST (R11-2007-050-03002-0 and R01-2007-000-11495).

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Entrapment of enzyme-linked magnetic nanoparticles within poly(ethylene glycol) hydrogel microparticles prepared by photopatterning

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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