Enterotoxigenic bacteroides fragilis infection exacerbates tumorigenesis in AOM/DSS mouse model

Soonjae Hwang, Chang Gun Lee, Minjeong Jo, Chan Oh Park, Sun Yeong Gwon, Samnoh Hwang, Hye Chin Yi, So Yeon Lee, Yong Bin Eom, Baktiar Karim, Ki Jong Rhee

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


The azoxymethane (AOM)/dextran sulfate sodium (DSS) murine model is commonly used to study colitis-associated cancer. The human commensal bacterium, enterotoxigenic Bacteroides fragilis (ETBF) secretes the Bacteroides fragilis toxin (BFT) which is necessary and sufficient to cause colitis. We report that BALB/c mice infected with WT-ETBF and administered three cycles of AOM/DSS developed numerous, large-sized polyps predominantly in the colorectal region. In addition, AOM/DSS-treated BALB/c mice orally inoculated with wild-type nontoxigenic Bacteroides fragilis (WT-NTBF) overexpressing bft (rETBF) developed numerous polyps whereas mice infected with WT-NTBF overexpressing a biologically inactive bft (rNTBF) did not promote polyp formation. Unexpectedly, the combination of AOM+ETBF did not induce polyp formation whereas ETBF+DSS did induce polyp development in a subset of BALB/c mice. In conclusion, WT-ETBF promoted polyp development in AOM/DSS murine model with increased colitis in BALB/c mice. The model described herein provides an experimental platform for understanding ETBF-induced colonic tumorigenesis and studying colorectal cancer in wild-type mice.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalInternational Journal of Medical Sciences
Issue number2
Publication statusPublished - 2020

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All Science Journal Classification (ASJC) codes

  • General Medicine


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