Enhancing guided bone regeneration with cross-linked collagen-conjugated xenogeneic bone blocks and membrane fixation: A preclinical in vivo study

Yin Zhe An, Young Woo Song, Daniel S. Thoma, Franz J. Strauss, Jung Seok Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To determine whether combining cross-linked (CL) collagen-integrated xenogeneic bone blocks stabilized with the fixation of resorbable collagen membranes (CM) can enhance guided bone regeneration (GBR) in the overaugmented calvarial defect model. Materials and Methods: Four circular defects with a diameter of 8 mm were prepared in the calvarium of 13 rabbits. Defects were randomly assigned to receive one of the following treatments: (i) non-cross-linked (NCL) porcine-derived collagen-embedded bone block covered by a CM without fixation (NCL + unfix group); (ii) NCL bone block covered by CM with fixation using bone-tack (NCL + fix group); (iii) cross-linked (CL) porcine-derived collagen-embedded bone block covered by CM without fixation (CL + unfix group); and (iv) CL bone block covered by CM with fixation using bone-tack fixation (CL + fix group). The efficacy of GBR was assessed through histological and molecular analyses after 2 and 8 weeks. Results: At 2 weeks, there were no significant differences in histologically measured areas of newly formed bone among the groups. At 8 weeks, however, the CL + fix group exhibited a larger area of new bone (5.08 ± 1.09 mm2, mean ± standard deviation) compared to the NCL + unfix (1.62 ± 0.42 mm2; p <.0083), NCL + fix (3.97 ± 1.39 mm2) and CL + unfix (2.55 ± 1.04 mm2) groups. Additionally, the expression levels of tumour necrosis factor-alpha, fibroblast growth factor-2, vascular endothelial growth factor, osteocalcin and calcitonin receptor were significantly higher in the CL + fix group compared to the other three groups (p <.0083). Conclusion: Cross-linked bone blocks stabilized with collagen membrane fixation can significantly enhance GBR.

Original languageEnglish
Pages (from-to)1226-1239
Number of pages14
JournalClinical Oral Implants Research
Volume35
Issue number10
DOIs
Publication statusPublished - 2024 Oct

Bibliographical note

Publisher Copyright:
© 2024 The Author(s). Clinical Oral Implants Research published by John Wiley & Sons Ltd.

All Science Journal Classification (ASJC) codes

  • Oral Surgery

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